Legumes symbioses: Absence of Nod genes in photosynthetic bradyrhizobia

Eric Giraud; Lionel Moulin; David Vallenet; Valérie Barbe; Eddie Cytryn; Jean Christophe Avarre; Marianne Jaubert; Damien Simon; Fabienne Cartieaux; Yves Prin; Gilles Bena; Laura Hannibal; Joel Fardoux; Mila Kojadinovic; Laurie Vuillet; Aurélie Lajus; Stéphane Cruveiller; Zoe Rouy; Sophie Mangenot; Béatrice Segurens; Carole Dossat; William L. Franck; Woo Suk Chang; Elizabeth Saunders; David Bruce; Paul Richardson; Philippe Normand; Bernard Dreyfus; David Pignol; Gary Stacey; David Emerich; André Verméglio; Claudine Médigue; Michael Sadowsky

doi:10.1126/science.1139548

Legumes symbioses: Absence of Nod genes in photosynthetic bradyrhizobia

Eric Giraud, Lionel Moulin, David Vallenet, Valérie Barbe, Eddie Cytryn, Jean Christophe Avarre, Marianne Jaubert, Damien Simon, Fabienne Cartieaux, Yves Prin, Gilles Bena, Laura Hannibal, Joel Fardoux, Mila Kojadinovic, Laurie Vuillet, Aurélie Lajus, Stéphane Cruveiller, Zoe Rouy, Sophie Mangenot, Béatrice SegurensCarole Dossat, William L. Franck, Woo Suk Chang, Elizabeth Saunders, David Bruce, Paul Richardson, Philippe Normand, Bernard Dreyfus, David Pignol, Gary Stacey, David Emerich, André Verméglio, Claudine Médigue, Michael Sadowsky

Soil, Water, and Climate

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Abstract

Leguminous plants (such as peas and soybeans) and rhizobial soil bacteria are symbiotic partners that communicate through molecular signaling pathways, resulting in the formation of nodules on legume roots and occasionally stems that house nitrogen-fixing bacteria. Nodule formation has been assumed to be exclusively initiated by the binding of bacterial, host-specific lipochito-oligosaccharidic Nod factors, encoded by the nodABC genes, to kinase-like receptors of the plant. Here we show by complete genome sequencing of two symbiotic, photosynthetic, Bradyrhizobium strains, BTAi1 and ORS278, that canonical nodABC genes and typical lipochito-oligosaccharidic Nod factors are not required for symbiosis in some legumes. Mutational analyses indicated that these unique rhizobia use an alternative pathway to initiate symbioses, where a purine derivative may play a key role in triggering nodule formation.

Original language	English (US)
Pages (from-to)	1307-1312
Number of pages	6
Journal	Science
Volume	316
Issue number	5829
DOIs	https://doi.org/10.1126/science.1139548
State	Published - Jun 1 2007

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Giraud, E., Moulin, L., Vallenet, D., Barbe, V., Cytryn, E., Avarre, J. C., Jaubert, M., Simon, D., Cartieaux, F., Prin, Y., Bena, G., Hannibal, L., Fardoux, J., Kojadinovic, M., Vuillet, L., Lajus, A., Cruveiller, S., Rouy, Z., Mangenot, S., ... Sadowsky, M. (2007). Legumes symbioses: Absence of Nod genes in photosynthetic bradyrhizobia. Science, 316(5829), 1307-1312. https://doi.org/10.1126/science.1139548

Giraud, E, Moulin, L, Vallenet, D, Barbe, V, Cytryn, E, Avarre, JC, Jaubert, M, Simon, D, Cartieaux, F, Prin, Y, Bena, G, Hannibal, L, Fardoux, J, Kojadinovic, M, Vuillet, L, Lajus, A, Cruveiller, S, Rouy, Z, Mangenot, S, Segurens, B, Dossat, C, Franck, WL, Chang, WS, Saunders, E, Bruce, D, Richardson, P, Normand, P, Dreyfus, B, Pignol, D, Stacey, G, Emerich, D, Verméglio, A, Médigue, C & Sadowsky, M 2007, 'Legumes symbioses: Absence of Nod genes in photosynthetic bradyrhizobia', Science, vol. 316, no. 5829, pp. 1307-1312. https://doi.org/10.1126/science.1139548

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abstract = "Leguminous plants (such as peas and soybeans) and rhizobial soil bacteria are symbiotic partners that communicate through molecular signaling pathways, resulting in the formation of nodules on legume roots and occasionally stems that house nitrogen-fixing bacteria. Nodule formation has been assumed to be exclusively initiated by the binding of bacterial, host-specific lipochito-oligosaccharidic Nod factors, encoded by the nodABC genes, to kinase-like receptors of the plant. Here we show by complete genome sequencing of two symbiotic, photosynthetic, Bradyrhizobium strains, BTAi1 and ORS278, that canonical nodABC genes and typical lipochito-oligosaccharidic Nod factors are not required for symbiosis in some legumes. Mutational analyses indicated that these unique rhizobia use an alternative pathway to initiate symbioses, where a purine derivative may play a key role in triggering nodule formation.",

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AU - Franck, William L.

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AU - Richardson, Paul

AU - Normand, Philippe

AU - Dreyfus, Bernard

AU - Pignol, David

AU - Stacey, Gary

AU - Emerich, David

AU - Verméglio, André

AU - Médigue, Claudine

AU - Sadowsky, Michael

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Legumes symbioses: Absence of Nod genes in photosynthetic bradyrhizobia

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