TY - JOUR
T1 - Leukocyte chemotaxis under agarose
T2 - Manipulations of serum and plasma before incorporation into agarose can influence cell movement
AU - Dahl, Mark V
AU - Lindroos, William E.
N1 - Funding Information:
I Supported by USPHS Grant No. 5-T32AMO7165.
PY - 1979/9/24
Y1 - 1979/9/24
N2 - In the migration under agarose assay for measurement of polymorphonuclear leukocyte chemotaxis and random migration, the agarose gel ordinarily contains 10% human serum or plasma. Influences of serum and plasma on cell movement using this assay were defined. Utilizing plasma as protein source allowed more random migration and chemotaxis than when serum was used. Heat inactivation of both serum and plasma decreased random migration and chemotaxis. If heating at 56°C was prolonged beyond 15-30 min, leukocyte movement was augmented. Excessive concentrations of platelets or platelet products, or heparin in agarose, also augmented migration, but storage of plasma or sera at various temperatures for 24 h did not appreciably affect migration. Large amounts of hemoglobin in agarose decreased chemotaxis, but the effect of trace amounts of hemolysis was negligible. Handling plasma or sera in standard ways prior to use in the under agarose assay may make this assay more reproducible and sensitive.
AB - In the migration under agarose assay for measurement of polymorphonuclear leukocyte chemotaxis and random migration, the agarose gel ordinarily contains 10% human serum or plasma. Influences of serum and plasma on cell movement using this assay were defined. Utilizing plasma as protein source allowed more random migration and chemotaxis than when serum was used. Heat inactivation of both serum and plasma decreased random migration and chemotaxis. If heating at 56°C was prolonged beyond 15-30 min, leukocyte movement was augmented. Excessive concentrations of platelets or platelet products, or heparin in agarose, also augmented migration, but storage of plasma or sera at various temperatures for 24 h did not appreciably affect migration. Large amounts of hemoglobin in agarose decreased chemotaxis, but the effect of trace amounts of hemolysis was negligible. Handling plasma or sera in standard ways prior to use in the under agarose assay may make this assay more reproducible and sensitive.
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U2 - 10.1016/0022-1759(79)90001-2
DO - 10.1016/0022-1759(79)90001-2
M3 - Article
C2 - 512358
AN - SCOPUS:0018291294
SN - 0022-1759
VL - 29
SP - 301
EP - 310
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 4
ER -