Ligand exchange chromatography of free amino acids on phosphated zirconium oxide supports

J. A. Blackwell, P. W. Carr

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Phosphated zirconium oxide particles loaded with copper ions are a mechanically superior alternative to resin based supports for ligand exchange separations of amino acids. They show good stability under acidic and alkaline conditions and afford different selectivity than resin based exchangers. Acidic amino acids are more strongly retained on this phase than on silica or resin based supports due to interactions with the underlying zirconia surface. These interactions occur despite rigorous phosphation of the zirconia particle. The operational aspects of amino acid separations on this material are examined and compared to conventional resin based models.

Original languageEnglish (US)
Pages (from-to)727-751
Number of pages25
JournalJournal of Liquid Chromatography
Volume15
Issue number5
DOIs
StatePublished - Apr 1 1992

Bibliographical note

Funding Information:
The authors would like to thank the National Institutes of Health and the National Science Foundation for supporting this

Funding Information:
JAB also acknowledges financial support from the Leading

Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.

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