Lipid-Detergent Phase Transitions During Detergent-Mediated Liposome Solubilization

Hanieh Niroomand, Guru A. Venkatesan, Stephen A. Sarles, Dibyendu Mukherjee, Bamin Khomami

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


We investigate the phase transition stages for detergent-mediated liposome solubilization of bio-mimetic membranes with the motivation of integrating membrane-bound Photosystem I into bio-hybrid opto-electronic devices. To this end, the interaction of two non-ionic detergents n-dodecyl-β-d-maltoside (DDM) and Triton X-100 (TX-100) with two types of phospholipids, namely DPhPC (1,2-diphytanoyl-sn-glycero-3-phosphocholine) and DPPG (1,2-dipalmitoyl-sn-glycero-3-phospho-(1′-rac-glycerol)), are examined. Specifically, solubilization processes for large unilamellar liposomes are studied with the aid of turbidity measurements, dynamic light scattering, and cryo-transmission electron microscopy imaging. Our results indicate that the solubilization process is well depicted by a three-stage model, wherein the lamellar-to-micellar transitions for DPhPC liposomes are dictated by the critical detergent/phospholipid ratios. The solubilization of DPhPC by DDM is devoid of formation of a “gel-like” phase. Furthermore, our results indicate that DDM is a stable candidate for DPhPC solubilization and proteoliposome formation. Finally, although the solubilization of DPPG with DDM indicated the familiar three-stage process, the same process with TX-100 indicate structural deformation of vesicles into complex network of kinetically trapped micro- and nanostructured arrangements of lipid bilayers.

Original languageEnglish (US)
Pages (from-to)523-538
Number of pages16
JournalJournal of Membrane Biology
Issue number4
StatePublished - Aug 1 2016


  • DPhPC and DPPG
  • Detergent-mediated liposome solubilization
  • Lamellar-to-complex structures
  • Lamellar-to-micellar transition
  • Transmission electron microscopy
  • n-dodecyl-β-d-maltoside (DDM) and Triton X-100 (TX-100)

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