Abstract
Background: Nuclear factor-κB (NF-κB) has been implicated in tumor cell proliferation and survival and in tumor angiogenesis. We sought to evaluate the effects of curcumin, an inhibitor of NF-κB, on a xenograft model of disseminated neuroblastoma. Methods: For in vitro studies, neuroblastoma cell lines NB1691, CHLA-20, and SK-N-AS were treated with various doses of liposomal curcumin. Disseminated neuroblastoma was established in vivo by tail vein injection of NB1691-luc cells into SCID mice, which were then treated with 50 mg/kg/day of liposomal curcumin 5 days/week intraperitoneally. Results: Curcumin suppressed NF-κB activation and proliferation of all neuroblastoma cell lines in vitro. In vivo, curcumin treatment resulted in a significant decrease in disseminated tumor burden. Curcumin-treated tumors had decreased NF-κB activity and an associated significant decrease in tumor cell proliferation and an increase in tumor cell apoptosis, as well as a decrease in tumor vascular endothelial growth factor levels and microvessel density. Conclusion: Liposomal curcumin suppressed neuroblastoma growth, with treated tumors showing a decrease in NF-κB activity. Our results suggest that liposomal curcumin may be a viable option for the treatment of neuroblastoma that works via inhibiting the NF-κB pathway.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 736-744 |
| Number of pages | 9 |
| Journal | Surgery |
| Volume | 151 |
| Issue number | 5 |
| DOIs | |
| State | Published - May 2012 |
Bibliographical note
Funding Information:Supported by the Assisi Foundation of Memphis , the U.S. Public Health Service Childhood Solid Tumor Program Project Grant No. CA23099 , the Cancer Center Support Grant No. 21766 from the National Cancer Institute , Grant No. CA133322 from National Cancer Institute , and by the American Lebanese Syrian Associated Charities (ALSAC) .
