TY - JOUR
T1 - Liquid Chromatographic Analysis of Di(2-ethylhexyl) Phthalate
T2 - Application to Pharmacokinetic Studies in the Mongrel Dog
AU - Haughey, David B.
AU - Elmquist, William F.
AU - Breutzmann, David A.
AU - Hemphill, D. Gary
AU - Constantini, Edward G.
PY - 1988/1
Y1 - 1988/1
N2 - A high-performance liquid chromatographic (HPLC) assay was developed for the determination of di(2-ethylhexyl) phthalate (DEHP) in serum or plasma. Plasma DEHP concentrations that were measured by HPLC in specimens obtained from hemodialysis patients were in good agreement with corresponding concentrations that were measured by gas chromatography with selected ion monitoring (GC-SIM) (r2 = 0.996). Plasma DEHP concentrations were measured after intravenous DEHP administration (1.2–4.4 mg DEHP/kg body weight) to determine the effect of bilateral ureteral ligation on DEHP elimination in the mongrel dog. DEHP plasma clearance (∼6.3 ml/min/kg), steady-state distribution volume (∼0.2l/kg), and terminal half-life (∼50 min) were unchanged in two dogs following bilateral ureteral ligation. DEHP terminal half-life and steady-state distribution volume were substantially smaller (25- to 70-fold) than reported previously in the rat or dog.
AB - A high-performance liquid chromatographic (HPLC) assay was developed for the determination of di(2-ethylhexyl) phthalate (DEHP) in serum or plasma. Plasma DEHP concentrations that were measured by HPLC in specimens obtained from hemodialysis patients were in good agreement with corresponding concentrations that were measured by gas chromatography with selected ion monitoring (GC-SIM) (r2 = 0.996). Plasma DEHP concentrations were measured after intravenous DEHP administration (1.2–4.4 mg DEHP/kg body weight) to determine the effect of bilateral ureteral ligation on DEHP elimination in the mongrel dog. DEHP plasma clearance (∼6.3 ml/min/kg), steady-state distribution volume (∼0.2l/kg), and terminal half-life (∼50 min) were unchanged in two dogs following bilateral ureteral ligation. DEHP terminal half-life and steady-state distribution volume were substantially smaller (25- to 70-fold) than reported previously in the rat or dog.
KW - di(2-ethylhexyl) phthalate
KW - liquid chromatographic (LC) analysis
KW - mongrel dog
KW - pharmacokinetics
KW - renal failure
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U2 - 10.1023/A:1015872309411
DO - 10.1023/A:1015872309411
M3 - Article
C2 - 3244602
AN - SCOPUS:0023870841
SN - 0724-8741
VL - 5
SP - 10
EP - 15
JO - Pharmaceutical Research: An Official Journal of the American Association of Pharmaceutical Scientists
JF - Pharmaceutical Research: An Official Journal of the American Association of Pharmaceutical Scientists
IS - 1
ER -