TY - JOUR
T1 - Liquid storage of unseparated human bone marrow. Evaluation of hematopoietic progenitors by clonal assay
AU - Lasky, L. C.
AU - McCullough, J.
AU - Zanjani, E. D.
PY - 1986
Y1 - 1986
N2 - Marrow from five donors was aspirated into a small volume of heparinized culture media, filtered, and stored in 10-ml nominal plastic bags at 4, 22, and 37°C, respectively, for 7 days with and without agitation. Samples were taken at 0, 1, 2, 3, and 7 days, respectively, for determination of cell numbers, trypan blue viability, and committed colony-forming unit-granulocyte-monocyte (CFU-GM) and erythroid burst-forming unit (BFU-E) and colony-forming unit (CFU-E), as well as multipotent (CFU-MIX), hematopoietic progenitors. Glucose and pH decreased with storage, most markedly at higher storage temperatures. Recovery of recovered cells exceeded 90 percent under all conditions at 7 days. Viability of committed and multipotent progenitors was maintained fairly well for 3 days at 4 and 22°C, but fell off rapidly after 1 day at 37°C. Recovery of progenitor cells stored 7 days was relatively poor. Best maintenance was achieved for 3 days using 4°C storage without agitation.
AB - Marrow from five donors was aspirated into a small volume of heparinized culture media, filtered, and stored in 10-ml nominal plastic bags at 4, 22, and 37°C, respectively, for 7 days with and without agitation. Samples were taken at 0, 1, 2, 3, and 7 days, respectively, for determination of cell numbers, trypan blue viability, and committed colony-forming unit-granulocyte-monocyte (CFU-GM) and erythroid burst-forming unit (BFU-E) and colony-forming unit (CFU-E), as well as multipotent (CFU-MIX), hematopoietic progenitors. Glucose and pH decreased with storage, most markedly at higher storage temperatures. Recovery of recovered cells exceeded 90 percent under all conditions at 7 days. Viability of committed and multipotent progenitors was maintained fairly well for 3 days at 4 and 22°C, but fell off rapidly after 1 day at 37°C. Recovery of progenitor cells stored 7 days was relatively poor. Best maintenance was achieved for 3 days using 4°C storage without agitation.
UR - http://www.scopus.com/inward/record.url?scp=0022536131&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022536131&partnerID=8YFLogxK
M3 - Article
C2 - 2873668
AN - SCOPUS:0022536131
SN - 0041-1132
VL - 26
SP - 331
EP - 334
JO - Transfusion
JF - Transfusion
IS - 4
ER -