TY - JOUR
T1 - Localization of rat liver group IIA phospholipase A2 in secretory pathways
T2 - Green fluorescent protein approach
AU - Zhang, Yingpei
AU - Lemasters, John
AU - Herman, Brian
PY - 2000/3
Y1 - 2000/3
N2 - Rat liver group IIA phospholipase A2 (PLA2, Enzyme Commission Number [E.C.] 3.1.1.4) was initially isolated from mitochondria. Since then it has been thought that it resides in mitochondria and that mitochondria are its main subcellular target. We cloned the cDNA sequence of rat group IIA PLA2 and fused its presequence (signal peptide) to enhanced green fluorescent protein (EGFP). In baby hamster kidney (BHK) cells transiently and stably expressing the fusion protein, the fluorescence does not localize in mitochondria, but rather in the secretory pathway. These results suggest that the plasma membrane, and not mitochondria, is the primary target of rat group IIA PLA2.
AB - Rat liver group IIA phospholipase A2 (PLA2, Enzyme Commission Number [E.C.] 3.1.1.4) was initially isolated from mitochondria. Since then it has been thought that it resides in mitochondria and that mitochondria are its main subcellular target. We cloned the cDNA sequence of rat group IIA PLA2 and fused its presequence (signal peptide) to enhanced green fluorescent protein (EGFP). In baby hamster kidney (BHK) cells transiently and stably expressing the fusion protein, the fluorescence does not localize in mitochondria, but rather in the secretory pathway. These results suggest that the plasma membrane, and not mitochondria, is the primary target of rat group IIA PLA2.
KW - Enhanced green fluorescent protein
KW - Group IIA phospholipase A
KW - Mitochondria
KW - Secretory pathway
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M3 - Article
AN - SCOPUS:0040784254
SN - 1431-9276
VL - 6
SP - 150
EP - 155
JO - Microscopy and Microanalysis
JF - Microscopy and Microanalysis
IS - 2
ER -