Long-term maintenance of a transgenic Catharanthus roseus hairy root line

Christie A.M. Peebles; Susan I. Gibson; Jacqueline V. Shanks; Ka Yiu San

doi:10.1021/bp0702166

Long-term maintenance of a transgenic Catharanthus roseus hairy root line

Christie A.M. Peebles, Susan I. Gibson, Jacqueline V. Shanks, Ka Yiu San

Plant and Microbial Biology

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Stably transformed transgenic hairy root cultures have the potential to be a valuable production platform for a variety of secondary metabolites. This study reports that a transgenic hairy root culture of Catharanthus roseus has been stably maintained for over 4.5 years. This culture carries a transgene that expresses the green fluorescent protein under the control of the glucocorticoid-inducible promoter. Genomic PCR confirmed the presence of the GFP insert within the hairy roots, and induction with dexamethasone caused a significant (p < 0.02) increase in GFP levels.

Original language	English (US)
Pages (from-to)	1517-1518
Number of pages	2
Journal	Biotechnology Progress
Volume	23
Issue number	6
DOIs	https://doi.org/10.1021/bp0702166
State	Published - Nov 2007

Access

10.1021/bp0702166

OpenUrl availability

Full text

Cite this

@article{feca8f51e79747baa11de0f8564e6e02,

title = "Long-term maintenance of a transgenic Catharanthus roseus hairy root line",

abstract = "Stably transformed transgenic hairy root cultures have the potential to be a valuable production platform for a variety of secondary metabolites. This study reports that a transgenic hairy root culture of Catharanthus roseus has been stably maintained for over 4.5 years. This culture carries a transgene that expresses the green fluorescent protein under the control of the glucocorticoid-inducible promoter. Genomic PCR confirmed the presence of the GFP insert within the hairy roots, and induction with dexamethasone caused a significant (p < 0.02) increase in GFP levels.",

author = "Peebles, {Christie A.M.} and Gibson, {Susan I.} and Shanks, {Jacqueline V.} and San, {Ka Yiu}",

year = "2007",

month = nov,

doi = "10.1021/bp0702166",

language = "English (US)",

volume = "23",

pages = "1517--1518",

journal = "Biotechnology Progress",

issn = "8756-7938",

publisher = "John Wiley and Sons Ltd",

number = "6",

}

TY - JOUR

T1 - Long-term maintenance of a transgenic Catharanthus roseus hairy root line

AU - Peebles, Christie A.M.

AU - Gibson, Susan I.

AU - Shanks, Jacqueline V.

AU - San, Ka Yiu

PY - 2007/11

Y1 - 2007/11

N2 - Stably transformed transgenic hairy root cultures have the potential to be a valuable production platform for a variety of secondary metabolites. This study reports that a transgenic hairy root culture of Catharanthus roseus has been stably maintained for over 4.5 years. This culture carries a transgene that expresses the green fluorescent protein under the control of the glucocorticoid-inducible promoter. Genomic PCR confirmed the presence of the GFP insert within the hairy roots, and induction with dexamethasone caused a significant (p < 0.02) increase in GFP levels.

AB - Stably transformed transgenic hairy root cultures have the potential to be a valuable production platform for a variety of secondary metabolites. This study reports that a transgenic hairy root culture of Catharanthus roseus has been stably maintained for over 4.5 years. This culture carries a transgene that expresses the green fluorescent protein under the control of the glucocorticoid-inducible promoter. Genomic PCR confirmed the presence of the GFP insert within the hairy roots, and induction with dexamethasone caused a significant (p < 0.02) increase in GFP levels.

UR - http://www.scopus.com/inward/record.url?scp=37149020754&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=37149020754&partnerID=8YFLogxK

U2 - 10.1021/bp0702166

DO - 10.1021/bp0702166

M3 - Article

C2 - 17900137

AN - SCOPUS:37149020754

SN - 8756-7938

VL - 23

SP - 1517

EP - 1518

JO - Biotechnology Progress

JF - Biotechnology Progress

IS - 6

ER -

Long-term maintenance of a transgenic Catharanthus roseus hairy root line

Abstract

Access

OpenUrl availability

Other files and links

Fingerprint

Cite this