Loss of AvrSr50 by somatic exchange in stem rust leads to virulence for Sr50 resistance in wheat

Jiapeng Chen; Narayana M. Upadhyaya; Diana Ortiz; Jana Sperschneider; Feng Li; Clement Bouton; Susan Breen; Chongmei Dong; Bo Xu; Xiaoxiao Zhang; Rohit Mago; Kim Newell; Xiaodi Xia; Maud Bernoux; Jennifer M. Taylor; Brian Steffenson; Yue Jin; Peng Zhang; Kostya Kanyuka; Melania Figueroa; Jeffrey G. Ellis; Robert F. Park; Peter N. Dodds

doi:10.1126/science.aao4810

Loss of AvrSr50 by somatic exchange in stem rust leads to virulence for Sr50 resistance in wheat

Jiapeng Chen, Narayana M. Upadhyaya, Diana Ortiz, Jana Sperschneider, Feng Li, Clement Bouton, Susan Breen, Chongmei Dong, Bo Xu, Xiaoxiao Zhang, Rohit Mago, Kim Newell, Xiaodi Xia, Maud Bernoux, Jennifer M. Taylor, Brian Steffenson, Yue Jin, Peng Zhang, Kostya Kanyuka, Melania FigueroaJeffrey G. Ellis, Robert F. Park, Peter N. Dodds

Plant Pathology

Research output: Contribution to journal › Article › peer-review

135 Scopus citations

Abstract

Race-specific resistance genes protect the global wheat crop from stem rust disease caused by Puccinia graminis f. sp. tritici (Pgt) but are often overcome owing to evolution of new virulent races of the pathogen. To understand virulence evolution in Pgt, we identified the protein ligand (AvrSr50) recognized by the Sr50 resistance protein. A spontaneous mutant of Pgt virulent to Sr50 contained a 2.5 mega-base pair loss-of-heterozygosity event. A haustorial secreted protein from this region triggers Sr50-dependent defense responses in planta and interacts directly with the Sr50 protein. Virulence alleles of AvrSr50 have arisen through DNA insertion and sequence divergence, and our data provide molecular evidence that in addition to sexual recombination, somatic exchange can play a role in the emergence of new virulence traits in Pgt.

Original language	English (US)
Pages (from-to)	1607-1610
Number of pages	4
Journal	Science
Volume	358
Issue number	6370
DOIs	https://doi.org/10.1126/science.aao4810
State	Published - Dec 22 2017

Bibliographical note

Funding Information:
Work described here was supported by the TwoBlades foundation, the Grains Research and Development Corporation (grants US00067 and CSP00161), University of Minnesota Lieberman-Okinow and Stakman Endowments, Experimental Station USDA– National Institute of Food and Agriculture Hatch Funds (project MIN-22-058), and the Biotechnology and Biological Sciences Research Council (project BB/P016855/1). J.C. was supported by the Judith and David Coffey Life Lab and the Plant Breeding Institute, University of Sydney. J.G.E. has a scientific advisory role with the TwoBlades foundation. Sequence data was deposited in the National Center for Biotechnology Information Sequencing Read Archive under bioprojects PRJNA396463 and PRJNA415866. DNA of the rust isolates used in this study is available from R.F.P. under a materials transfer agreement with the University of Sydney. We thank R. East and L. Ma for excellent technical assistance. Additional data are provided in the supplementary materials.

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Chen, J., Upadhyaya, N. M., Ortiz, D., Sperschneider, J., Li, F., Bouton, C., Breen, S., Dong, C., Xu, B., Zhang, X., Mago, R., Newell, K., Xia, X., Bernoux, M., Taylor, J. M., Steffenson, B., Jin, Y., Zhang, P., Kanyuka, K., ... Dodds, P. N. (2017). Loss of AvrSr50 by somatic exchange in stem rust leads to virulence for Sr50 resistance in wheat. Science, 358(6370), 1607-1610. https://doi.org/10.1126/science.aao4810

Chen, J, Upadhyaya, NM, Ortiz, D, Sperschneider, J, Li, F, Bouton, C, Breen, S, Dong, C, Xu, B, Zhang, X, Mago, R, Newell, K, Xia, X, Bernoux, M, Taylor, JM, Steffenson, B , Jin, Y, Zhang, P, Kanyuka, K, Figueroa, M, Ellis, JG, Park, RF & Dodds, PN 2017, 'Loss of AvrSr50 by somatic exchange in stem rust leads to virulence for Sr50 resistance in wheat', Science, vol. 358, no. 6370, pp. 1607-1610. https://doi.org/10.1126/science.aao4810

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title = "Loss of AvrSr50 by somatic exchange in stem rust leads to virulence for Sr50 resistance in wheat",

abstract = "Race-specific resistance genes protect the global wheat crop from stem rust disease caused by Puccinia graminis f. sp. tritici (Pgt) but are often overcome owing to evolution of new virulent races of the pathogen. To understand virulence evolution in Pgt, we identified the protein ligand (AvrSr50) recognized by the Sr50 resistance protein. A spontaneous mutant of Pgt virulent to Sr50 contained a 2.5 mega-base pair loss-of-heterozygosity event. A haustorial secreted protein from this region triggers Sr50-dependent defense responses in planta and interacts directly with the Sr50 protein. Virulence alleles of AvrSr50 have arisen through DNA insertion and sequence divergence, and our data provide molecular evidence that in addition to sexual recombination, somatic exchange can play a role in the emergence of new virulence traits in Pgt.",

author = "Jiapeng Chen and Upadhyaya, {Narayana M.} and Diana Ortiz and Jana Sperschneider and Feng Li and Clement Bouton and Susan Breen and Chongmei Dong and Bo Xu and Xiaoxiao Zhang and Rohit Mago and Kim Newell and Xiaodi Xia and Maud Bernoux and Taylor, {Jennifer M.} and Brian Steffenson and Yue Jin and Peng Zhang and Kostya Kanyuka and Melania Figueroa and Ellis, {Jeffrey G.} and Park, {Robert F.} and Dodds, {Peter N.}",

note = "Funding Information: Work described here was supported by the TwoBlades foundation, the Grains Research and Development Corporation (grants US00067 and CSP00161), University of Minnesota Lieberman-Okinow and Stakman Endowments, Experimental Station USDA– National Institute of Food and Agriculture Hatch Funds (project MIN-22-058), and the Biotechnology and Biological Sciences Research Council (project BB/P016855/1). J.C. was supported by the Judith and David Coffey Life Lab and the Plant Breeding Institute, University of Sydney. J.G.E. has a scientific advisory role with the TwoBlades foundation. Sequence data was deposited in the National Center for Biotechnology Information Sequencing Read Archive under bioprojects PRJNA396463 and PRJNA415866. DNA of the rust isolates used in this study is available from R.F.P. under a materials transfer agreement with the University of Sydney. We thank R. East and L. Ma for excellent technical assistance. Additional data are provided in the supplementary materials.",

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AU - Chen, Jiapeng

AU - Upadhyaya, Narayana M.

AU - Ortiz, Diana

AU - Sperschneider, Jana

AU - Li, Feng

AU - Bouton, Clement

AU - Breen, Susan

AU - Dong, Chongmei

AU - Xu, Bo

AU - Zhang, Xiaoxiao

AU - Mago, Rohit

AU - Newell, Kim

AU - Xia, Xiaodi

AU - Bernoux, Maud

AU - Taylor, Jennifer M.

AU - Steffenson, Brian

AU - Jin, Yue

AU - Zhang, Peng

AU - Kanyuka, Kostya

AU - Figueroa, Melania

AU - Ellis, Jeffrey G.

AU - Park, Robert F.

AU - Dodds, Peter N.

N1 - Funding Information: Work described here was supported by the TwoBlades foundation, the Grains Research and Development Corporation (grants US00067 and CSP00161), University of Minnesota Lieberman-Okinow and Stakman Endowments, Experimental Station USDA– National Institute of Food and Agriculture Hatch Funds (project MIN-22-058), and the Biotechnology and Biological Sciences Research Council (project BB/P016855/1). J.C. was supported by the Judith and David Coffey Life Lab and the Plant Breeding Institute, University of Sydney. J.G.E. has a scientific advisory role with the TwoBlades foundation. Sequence data was deposited in the National Center for Biotechnology Information Sequencing Read Archive under bioprojects PRJNA396463 and PRJNA415866. DNA of the rust isolates used in this study is available from R.F.P. under a materials transfer agreement with the University of Sydney. We thank R. East and L. Ma for excellent technical assistance. Additional data are provided in the supplementary materials.

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N2 - Race-specific resistance genes protect the global wheat crop from stem rust disease caused by Puccinia graminis f. sp. tritici (Pgt) but are often overcome owing to evolution of new virulent races of the pathogen. To understand virulence evolution in Pgt, we identified the protein ligand (AvrSr50) recognized by the Sr50 resistance protein. A spontaneous mutant of Pgt virulent to Sr50 contained a 2.5 mega-base pair loss-of-heterozygosity event. A haustorial secreted protein from this region triggers Sr50-dependent defense responses in planta and interacts directly with the Sr50 protein. Virulence alleles of AvrSr50 have arisen through DNA insertion and sequence divergence, and our data provide molecular evidence that in addition to sexual recombination, somatic exchange can play a role in the emergence of new virulence traits in Pgt.

AB - Race-specific resistance genes protect the global wheat crop from stem rust disease caused by Puccinia graminis f. sp. tritici (Pgt) but are often overcome owing to evolution of new virulent races of the pathogen. To understand virulence evolution in Pgt, we identified the protein ligand (AvrSr50) recognized by the Sr50 resistance protein. A spontaneous mutant of Pgt virulent to Sr50 contained a 2.5 mega-base pair loss-of-heterozygosity event. A haustorial secreted protein from this region triggers Sr50-dependent defense responses in planta and interacts directly with the Sr50 protein. Virulence alleles of AvrSr50 have arisen through DNA insertion and sequence divergence, and our data provide molecular evidence that in addition to sexual recombination, somatic exchange can play a role in the emergence of new virulence traits in Pgt.

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ER -

Loss of AvrSr50 by somatic exchange in stem rust leads to virulence for Sr50 resistance in wheat

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