Loss of immune homeostasis dictates SHIV rebound after stem-cell transplantation

Christopher W Peterson, Clarisse Benne, Patricia Polacino, Jasbir Kaur, Cristina E McAllister, Abdelali Filali-Mouhim, Willi Obenza, Tiffany A Pecor, Meei-Li Huang, Audrey Baldessari, Robert D Murnane, Ann E Woolfrey, Keith R Jerome, Shiu-Lok Hu, Nichole R Klatt, Stephen DeRosa, Rafick P Sékaly, Hans-Peter Kiem

Research output: Contribution to journalArticlepeer-review


The conditioning regimen used as part of the Berlin patient's hematopoietic cell transplant likely contributed to his eradication of HIV infection. We studied the impact of conditioning in simian-human immunodeficiency virus-infected (SHIV-infected) macaques suppressed by combination antiretroviral therapy (cART). The conditioning regimen resulted in a dramatic, but incomplete depletion of CD4+ and CD8+ T cells and CD20+ B cells, increased T cell activation and exhaustion, and a significant loss of SHIV-specific Abs. The disrupted T cell homeostasis and markers of microbial translocation positively correlated with an increased viral rebound after cART interruption. Quantitative viral outgrowth and Tat/rev-induced limiting dilution assays showed that the size of the latent SHIV reservoir did not correlate with viral rebound. These findings identify perturbations of the immune system as a mechanism for the failure of autologous transplantation to eradicate HIV. Thus, transplantation strategies may be improved by incorporating immune modulators to prevent disrupted homeostasis, and gene therapy to protect transplanted cells.

Original languageEnglish (US)
Pages (from-to)e91230
JournalJCI Insight
Issue number4
StatePublished - Feb 23 2017
Externally publishedYes


  • Animals
  • Anti-Retroviral Agents/therapeutic use
  • CD4-Positive T-Lymphocytes/immunology
  • CD8-Positive T-Lymphocytes/immunology
  • HIV Infections/drug therapy
  • HIV-1/radiation effects
  • Hematopoietic Stem Cell Transplantation/methods
  • Homeostasis/radiation effects
  • Lentivirus Infections/drug therapy
  • Macaca nemestrina
  • Simian Acquired Immunodeficiency Syndrome/drug therapy
  • Simian Immunodeficiency Virus/radiation effects
  • Transplantation Conditioning/methods
  • Transplantation, Autologous
  • Viral Load/radiation effects
  • Whole-Body Irradiation

PubMed: MeSH publication types

  • Journal Article
  • Research Support, N.I.H., Extramural


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