Low Dose Gamma Irradiation to Reduce Pathogenic Vibrios in Live Oysters (Crassostrea virginica)

Linda Andrews, Michael Jahncke, Kumar Mallikarjunan

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

Pathogenic strains of Vibrio (Vibrio vulnificus and V. parahaemolyticus), natural inhabitants of estuarine and ocean environments, can cause serious illness and death in susceptible persons from consumption of raw half-shell oysters. Objectives of this study were (1) to establish the irradiation dose needed to reduce pathogenic vibrios to nondetectable levels and (2) to determine consumer's ability to differentiate between irradiated and control oysters. Live oysters, Crassostrea virginica, with naturally incurred and artificially inoculated pathogenic vibrios, were exposed to 0-3 kGy dose Cobalt-60 gamma radiation. Vibrio vulnificus (MO-624) was reduced from 10 6 cfu/g oyster meat to nondetectable levels (< 3 mpn/g oyster meat) with 0.75-1.0 kGy irradiation exposure. Vibrio parahaemolyticus, 03:K6 (TX-2103), required 1.0-1.5 kGy for reduction to nondetectable levels. Using triangle difference testing, sensory panelists were asked to identify differences between treated (1 kGy) and untreated oysters. Sensory difference tests, triangle method, by 146 volunteers confirmed that panelists, many of whom worked in the seafood industry, were unable to distinguish non-irradiated from irradiated oysters (p < 0.001).

Original languageEnglish (US)
Pages (from-to)71-82
Number of pages12
JournalJournal of Aquatic Food Product Technology
Volume12
Issue number3
DOIs
StatePublished - 2003

Bibliographical note

Funding Information:
This work is a result of research sponsored in part by the National Oceanic and Atmospheric Administration, Department of Commerce under Grant #NA860039 GMO9920, the Mississippi-Alabama Sea Grant Consortium,Virginia Sea Grant Consortium, Mississippi State University, and Virginia Polytechnical Institute. The views expressed herein are those of the authors and do not necessarily reflect the views of NOAA or its sub agencies. This work is MAFES #J10335.

Keywords

  • Irradiation
  • Oysters
  • Vibrios

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