Lymphocyte aggregation in response to adrenergic stimulation

D. E. Hammerschmidt, C. Jeanneret, M. Husak, M. Lobell, H. S. Jacob

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


A nonanemic chronic lymphocytic leukemia patient with nearly 500,000 lymphocytes/μL underwent leukapheresis when she presented with CNS symptoms and retinal vascular engorgement. Respiratory distress developed during the cell separator run, which led us to ask whether the procedure could have changed the adhesive properties of her cells. C5a(desar ginine') N-f-Met-Leu-Phe, adenosine disphosphate, and collagen all failed to aggregate her lymphocytes in vitro, but arachidonic acid, excess free calcium, and 4 μmol/L, epinephrine did aggregate the cells. Arachidonate-induced aggregation appeared to be a toxic phenomenon: the ED50 for aggregation was statistically indistinguishable from that for cytotoxicity, and aspirin only mildly blunted the response. In contrast, epinephrine-induced aggregation was not associated with lactic dehydrogenase release or the loss of trypan blue exclusion and was blunted by propranolol; a radiopindolol-binding studies confirmed the presence of β-adrenergic receptor. There were approximately 3,000 receptors/cell, with no statistically significant difference between normal and chronic lymphocytic leukemia B cells or between B cells and T cells (separated by rosetting techniques). The K(d) for the B cells' receptor, however, was less than that for T cells by a factor of ten (P < .01). We conclude that B cells may aggregate when stimulated and that they - like T cells - have β-adrenergic receptors. Adrenergically mediated changes in B cell adhesiveness may play a role in regulating lymphocyte traffic; in the rare patient with truly enormous B cell counts, we postulate that they may be an occasional cause of morbidity.

Original languageEnglish (US)
Pages (from-to)1470-1474
Number of pages5
Issue number5
StatePublished - 1988


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