TY - JOUR
T1 - Lysine 2-hydroxyisobutyrylation is a widely distributed active histone mark
AU - Dai, Lunzhi
AU - Peng, Chao
AU - Montellier, Emilie
AU - Lu, Zhike
AU - Chen, Yue
AU - Ishii, Haruhiko
AU - Debernardi, Alexandra
AU - Buchou, Thierry
AU - Rousseaux, Sophie
AU - Jin, Fulai
AU - Sabari, Benjamin R.
AU - Deng, Zhiyou
AU - Allis, C. David
AU - Ren, Bing
AU - Khochbin, Saadi
AU - Zhao, Yingming
N1 - Funding Information:
S.K.’s group research is supported by Agence Nationale de la Recherche EpiSperm and Institut National du Cancer funds. E.M. was supported by a three-year grant from the French Ministry of Research and an Association pour la Recherche sur le Cancer fellowship for her fourth-year PhD.
PY - 2014/5
Y1 - 2014/5
N2 - We report the identification of a new type of histone mark, lysine 2-hydroxyisobutyrylation (K hib), and identify the mark at 63 human and mouse histone K hib sites, including 27 unique lysine sites that are not known to be modified by lysine acetylation (K ac) and lysine crotonylation (K cr). This histone mark was initially identified by MS and then validated by chemical and biochemical methods. Histone K hib shows distinct genomic distributions from histone K ac or histone K cr during male germ cell differentiation. Using chromatin immunoprecipitation sequencing, gene expression analysis and immunodetection, we show that in male germ cells, H4K8 hib is associated with active gene transcription in meiotic and post-meiotic cells. In addition, H4K8 ac -associated genes are included in and constitute only a subfraction of H4K8 hib -labeled genes. The histone K hib mark is conserved and widely distributed, has high stoichiometry and induces a large structural change. These findings suggest its critical role on the regulation of chromatin functions.
AB - We report the identification of a new type of histone mark, lysine 2-hydroxyisobutyrylation (K hib), and identify the mark at 63 human and mouse histone K hib sites, including 27 unique lysine sites that are not known to be modified by lysine acetylation (K ac) and lysine crotonylation (K cr). This histone mark was initially identified by MS and then validated by chemical and biochemical methods. Histone K hib shows distinct genomic distributions from histone K ac or histone K cr during male germ cell differentiation. Using chromatin immunoprecipitation sequencing, gene expression analysis and immunodetection, we show that in male germ cells, H4K8 hib is associated with active gene transcription in meiotic and post-meiotic cells. In addition, H4K8 ac -associated genes are included in and constitute only a subfraction of H4K8 hib -labeled genes. The histone K hib mark is conserved and widely distributed, has high stoichiometry and induces a large structural change. These findings suggest its critical role on the regulation of chromatin functions.
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U2 - 10.1038/nchembio.1497
DO - 10.1038/nchembio.1497
M3 - Article
C2 - 24681537
AN - SCOPUS:84899473489
SN - 1552-4450
VL - 10
SP - 365
EP - 370
JO - Nature Chemical Biology
JF - Nature Chemical Biology
IS - 5
ER -