Lysozyme adsorption onto mesoporous materials: Effect of pore geometry and stability of adsorbents

Ajayan Vinu, Masahiko Miyahara, Kazi Zakir Hossain, Motoi Takahashi, Veerappan Vaithilingam Balasubramanian, Toshiyuki Mori, Katsuhiko Ariga

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

In this paper, adsorption of lysozyme onto two kinds of mesoporous adsorbents (KIT-5 and AISBA-15) has been investigated and the results on the effects of pore geometry and stability of the adsorbents are also discussed. The KIT-5 mesoporous silica materials possess cage-type pore geometry while the AISBA-15 adsorbent has mesopores of cylindrical type with rather large diameter (9.7 nm). Adsorption of lysozyme onto AISBA-15 aluminosilicate obeys a Langmuir isotherm, resulting in pore occupation of 25 to 30%. In contrast, the KIT-5 adsorbents showed very small adsorption capacities for the lysozyme adsorption, typically falling in 6 to 13% of pore occupation. The cage-type KIT-5 adsorbents have narrow channel (4 to 6 nm) where penetration of the lysozyme (3 × 3 × 4.5 nm) might be restricted. The KIT-5 adsorbent tends to collapse after long-time immersion in water, as indicated by XRD patterns, while the AISBA-15 adsorbent retains its regular structure even after immersion in basic water for 4 days. These facts confirm superiority of the AISBA-15 as an adsorbent as compared with the KIT-5 mesoporous silicates. This research strikingly demonstrates the selection of mesoporous materials is crucial to achieve efficient immobilization of biomaterials in aqueous environment.

Original languageEnglish (US)
Pages (from-to)828-832
Number of pages5
JournalJournal of Nanoscience and Nanotechnology
Volume7
Issue number3
DOIs
StatePublished - Mar 1 2007

Keywords

  • AISBA-15
  • Adsorption
  • KIT-5
  • Lysozyme
  • Mesoporous materials

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