Machine Learning Based Real-Time Image-Guided Cell Sorting and Classification

Yi Gu, Alex Ce Zhang, Yuanyuan Han, Jie Li, Clark C Chen, Yu Hwa Lo

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Cell classification based on phenotypical, spatial, and genetic information greatly advances our understanding of the physiology and pathology of biological systems. Technologies derived from next generation sequencing and fluorescent activated cell sorting are cornerstones for cell- and genomic-based assays supporting cell classification and mapping. However, there exists a deficiency in technology space to rapidly isolate cells based on high content image information. Fluorescence-activated cell sorting can only resolve cell-to-cell variation in fluorescence and optical scattering. Utilizing microfluidics, photonics, computation microscopy, real-time image processing and machine learning, we demonstrate an image-guided cell sorting and classification system possessing the high throughput of flow cytometer and high information content of microscopy. We demonstrate the utility of this technology in cell sorting based on (1) nuclear localization of glucocorticoid receptors, (2) particle binding to the cell membrane, and (3) DNA damage induced γ-H2AX foci.

Original languageEnglish (US)
Pages (from-to)499-509
Number of pages11
JournalCytometry Part A
Volume95
Issue number5
DOIs
StatePublished - May 2019

Keywords

  • image guided cell sorting
  • imaging flow cytometry
  • machine learning
  • microfluidic

PubMed: MeSH publication types

  • Journal Article
  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

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