Valvular heart disease is the third most common cardiovascular disorder in the USA Calcification of the aortic valve is a common cause of the problem and often requires surgical replacement Extracellular matrix deposition associated with calcification and cell proliferation can be a normal tissue response to injury. Macrophages are prominent cells in response to injury and produce a variety of growth factors which increase cell proliferation and matrix. The purpose of this study was to examine the effects of macrophage conditioned media on porcine valvular subendothelial cells. Bone sialoprotein (BSP) is an Arg-GlyAsp containing acidic phosphoprotein associated with mineralized tissue. Porcine valvular subendothelial cells (SEC) were isolated by an expiant technique. In the presence of macrophage conditioned media, subendothelial proliferation (thymidine uptake) increased 400% over control, (p<0.005). Increased BSP was detected by immunocytochemistry and was increased by ELISA 30% over control, (p<0.002). Northern blot analysis for BSP mRNA was increased after 12 and 24 h exposure to conditioned media compared to control media. These observations provide further understanding into the cell - cell interactions and matrix protein production in valvular subendothelial cells and suggest that the macrophage plays an integral role in the induction of changes associated with valvular calcificatioa.
|Original language||English (US)|
|State||Published - 1996|