Macrophage inflammatory proteins in cytomegalovirus-related inner ear injury

Scott A. Schraff, Mark R. Schleiss, David K. Brown, Jareen Meinzen-Derr, K. Yeon Choi, John H. Greinwald, Daniel I. Choo

Research output: Contribution to journalArticlepeer-review

18 Scopus citations


Objective: Inner ear inflammation triggered by CMV infection may play a role in CMV-related auditory pathogenesis. The purpose of the study was to determine if a virally encoded macrophage inflammatory protein played a role in CMV-related hearing loss. Design: Mutagenesis was performed with deletion of a guinea pig CMV macrophage inflammatory protein. Intracochlear inoculations were performed on three groups of animals (n = 18). Group 1 received sterile viral media, Group 2 received wild-type CMV virus, and Group 3 received "knockout" (KO) virus with a deleted immunomodulation gene. Baseline and postinoculation ABRs were obtained. ELISA and PCR were performed and temporal bones examined. Subjects: Eighteen guinea pigs. Results: The KO group had significantly better hearing than the WT group. There were no significant differences between the KO and sham groups. The WT group had significant hearing loss at all frequencies. Inflammation and fibrosis were noted in the WT temporal bones only. Conclusions: Virally encoded macrophage inflammatory proteins appear to play a significant role in CMV-related hearing loss.

Original languageEnglish (US)
Pages (from-to)612-618
Number of pages7
JournalOtolaryngology - Head and Neck Surgery
Issue number4
StatePublished - Oct 2007

Bibliographical note

Funding Information:
This study was funded by a 2005 American Academy of Otolaryngology–Head and Neck Surgery Resident Research Grant. This research was also supported by RO1 grant 5R01HD044864-05.

Copyright 2008 Elsevier B.V., All rights reserved.

Fingerprint Dive into the research topics of 'Macrophage inflammatory proteins in cytomegalovirus-related inner ear injury'. Together they form a unique fingerprint.

Cite this