Mycobacterium avium complex (MAC) infection is the most common disseminated opportunistic infection encountered in patients with AIDS. We have studied the ability of specific Mycobacterium avium (MA) antigen to stimulate human monocyte-derived macrophages (MDM) to produce tumor necrosis factor-α (TNF-α). MDM stimulated with MA sonicate, MA 68 kDa and MA 48-52 kDa antigens were found to produce TNF-α in a dose-dependent manner. Reverse transcriptase-polymerase chain reaction analysis of mRNA extracts from antigen-stimulated MDM indicated that TNF-α mRNA expression was of brief duration and the time point of peak TNF-α mRNA levels was found to be antigen-specific. A significant difference in TNF-α production in response to MA 48-52 kDa antigen and M. bovis 65 kDa antigen was observed between MDM from normal and HIV positive individuals.
Bibliographical noteFunding Information:
We wish to thank Dr. P. Sriramarao, La Jolla Institute for Experimental Medicine, La Jolla, CA, for help with the RTPCR studies. This study was supportedb y Grant 5ROlAI33827 from the National Institutes of Health.
- HIV-positive patients
- Monocyte-derived macrophage
- Mycobacterium avium antigen
- Tumor necrosis factor-α