Male donor-derived cells in the brains of female sex-mismatched bone marrow transplant recipients: A Y-chromosome specific in situ hybridization study

E. R. Unger, Joo Ho Sung, J. C. Manivel, M. L. Chenggis, B. R. Blazar, W. Krivit

Research output: Contribution to journalArticlepeer-review

148 Scopus citations

Abstract

In five female bone marrow transplant (BMT) recipients of sex-mismatched donor marrow, Y-chromosome specific in situ hybridization was performed on formalin-fixed, paraffin-embedded sections of the medulla to detect the male donor marrow-derived cells. Y-chromosome-bearing cells (Y-cells), thereby donor-derived, were matched with leukocyte common antigen (LCA)-reactive cells in adjacent sections immunostained with anti-LCA antibody. Y-cells included mononuclear leukocytes (MNL) within the vessel lumen and infiltrating the perivascular space and parenchyma, and “perivascular cells.” We have, therefore, concluded that donor marrow-derived MNL, though limited in number, do enter the normal-appearing brain and can transform to “perivascular cells” in human BMT recipients. It remains, however, to be confirmed whether MNL entering the normal adult CNS parenchyma transform to ramified microglia.

Original languageEnglish (US)
Pages (from-to)460-470
Number of pages11
JournalJournal of neuropathology and experimental neurology
Volume52
Issue number5
DOIs
StatePublished - Sep 1993

Keywords

  • Cns
  • Female BMT recipients
  • In situ hybridization
  • Male donor cells
  • Y-chromosome

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