Malignant transformation in human chondrosarcoma cells supported by telomerase activation and tumor suppressor inactivation

James A. Martin, Erin Forest, Joel A. Block, Aloysius J. Klingelhutz, Brent Whited, Steven Gitelis, Andrew Wilkey, Joseph A. Buckwalter

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Human chondrosarcomas do not respond to current chemotherapies or radiation therapy, and their size and histological appearance do not reliably predict the risk of local recurrence and metastases, making selection of surgical treatment difficult. Identifying mechanisms responsible for the proliferation and invasive behavior of these tumors would be of immense clinical value. We hypothesized that telomerase expression is one of these mechanisms. We detected telomerase expression in 7 of 16 chondrosarcomas, but cells cultured from telomerase-negative chondrosarcomas acquired strong telomerase activity and lost tumor suppressor activity after their establishment in culture. These changes were associated with accelerated indefinite cell proliferation, morphological transition, and increased invasive activity, indicating that telomerase activation and loss of cell cycle control leads to the emergence of aggressive cells from chondrosarcoma cell populations. These observations may lead to better understanding of the factors responsible for malignant transformation, local recurrence, and metastases of cartilage neoplasms.

Original languageEnglish (US)
Pages (from-to)397-407
Number of pages11
JournalCell Growth and Differentiation
Volume13
Issue number9
StatePublished - Sep 1 2002
Externally publishedYes

Fingerprint Dive into the research topics of 'Malignant transformation in human chondrosarcoma cells supported by telomerase activation and tumor suppressor inactivation'. Together they form a unique fingerprint.

Cite this