Manipulating cultured mammalian cells for mitosis research

Charles A. Day; Alyssa Langfald; Edward H. Hinchcliffe

doi:10.1016/bs.mcb.2020.02.001

Manipulating cultured mammalian cells for mitosis research

Charles A. Day, Alyssa Langfald, Edward H. Hinchcliffe

Hormel Institute

Research output: Chapter in Book/Report/Conference proceeding › Chapter

1 Scopus citations

Abstract

The study of mitosis has always relied on bulk-preparation biochemistry techniques (Mazia & Dan, 1952), but very early on lent itself to living, single cell microscopic techniques (Inoue, 1953; Taylor, 1959). Here we describe several of the methods used by our lab to study cell division in living cultured cells, including cold-induced mitotic arrest, cold-induced chromosome missegregation, same-cell live and fixed cell imaging, and microinjection of inactivating antibodies. We detail our imaging system based on an upright fluorescent microscope and spinning disk confocal, as well as the customized “HEKS” metal support slide imaging chambers.

Original language	English (US)
Title of host publication	Methods in Cell Biology
Editors	Phong Tran
Publisher	Academic Press Inc.
Pages	43-61
Number of pages	19
ISBN (Print)	9780128200087
DOIs	https://doi.org/10.1016/bs.mcb.2020.02.001
State	Published - 2020

Publication series

Name	Methods in Cell Biology
Volume	158
ISSN (Print)	0091-679X

Bibliographical note

Funding Information:
Work in the author's lab is supported by grants from NIH (HL125353) and the DoD CDMRP (CA171071 and CA130436), and support from the I.J. Holton Trust and the Hormel Foundation.

Publisher Copyright:
© 2020 Elsevier Inc.

Keywords

Cell culture
Cell cycle
Chilling
Chromosomes
Cold-dependent
Microinjection
Microscopy
Microtubule
Spindle
Spinning disk

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title = "Manipulating cultured mammalian cells for mitosis research",

abstract = "The study of mitosis has always relied on bulk-preparation biochemistry techniques (Mazia & Dan, 1952), but very early on lent itself to living, single cell microscopic techniques (Inoue, 1953; Taylor, 1959). Here we describe several of the methods used by our lab to study cell division in living cultured cells, including cold-induced mitotic arrest, cold-induced chromosome missegregation, same-cell live and fixed cell imaging, and microinjection of inactivating antibodies. We detail our imaging system based on an upright fluorescent microscope and spinning disk confocal, as well as the customized “HEKS” metal support slide imaging chambers.",

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note = "Funding Information: Work in the author's lab is supported by grants from NIH (HL125353) and the DoD CDMRP (CA171071 and CA130436), and support from the I.J. Holton Trust and the Hormel Foundation. Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",

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AU - Langfald, Alyssa

AU - Hinchcliffe, Edward H.

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AB - The study of mitosis has always relied on bulk-preparation biochemistry techniques (Mazia & Dan, 1952), but very early on lent itself to living, single cell microscopic techniques (Inoue, 1953; Taylor, 1959). Here we describe several of the methods used by our lab to study cell division in living cultured cells, including cold-induced mitotic arrest, cold-induced chromosome missegregation, same-cell live and fixed cell imaging, and microinjection of inactivating antibodies. We detail our imaging system based on an upright fluorescent microscope and spinning disk confocal, as well as the customized “HEKS” metal support slide imaging chambers.

KW - Cell culture

KW - Cell cycle

KW - Chilling

KW - Chromosomes

KW - Cold-dependent

KW - Microinjection

KW - Microscopy

KW - Microtubule

KW - Spindle

KW - Spinning disk

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SN - 9780128200087

T3 - Methods in Cell Biology

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BT - Methods in Cell Biology

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PB - Academic Press Inc.

ER -

Manipulating cultured mammalian cells for mitosis research

Abstract

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Bibliographical note

Keywords

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