@inbook{4d3c32ef43c14b8ab347310fe8b13a60,
title = "Manipulating cultured mammalian cells for mitosis research",
abstract = "The study of mitosis has always relied on bulk-preparation biochemistry techniques (Mazia & Dan, 1952), but very early on lent itself to living, single cell microscopic techniques (Inoue, 1953; Taylor, 1959). Here we describe several of the methods used by our lab to study cell division in living cultured cells, including cold-induced mitotic arrest, cold-induced chromosome missegregation, same-cell live and fixed cell imaging, and microinjection of inactivating antibodies. We detail our imaging system based on an upright fluorescent microscope and spinning disk confocal, as well as the customized “HEKS” metal support slide imaging chambers.",
keywords = "Cell culture, Cell cycle, Chilling, Chromosomes, Cold-dependent, Microinjection, Microscopy, Microtubule, Spindle, Spinning disk",
author = "Day, {Charles A.} and Alyssa Langfald and Hinchcliffe, {Edward H.}",
year = "2020",
doi = "10.1016/bs.mcb.2020.02.001",
language = "English (US)",
isbn = "9780128200087",
series = "Methods in Cell Biology",
publisher = "Academic Press Inc.",
pages = "43--61",
editor = "Phong Tran",
booktitle = "Methods in Cell Biology",
address = "United States",
}