Markers linked to wheat stem rust resistance gene Sr11 effective to Puccinia graminis f. sp. tritici race TKTTF

Jayaveeramuthu Nirmala, Shiaoman Chao, Pablo Olivera, Ebrahiem M. Babiker, Bekele Abeyo, Zerihun Tadesse, Muhammad Imtiaz, Luther Talbert, Nancy K. Blake, Eduard Akhunov, Michael O. Pumphrey, Yue Jin, Matthew N. Rouse

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Wheat stem rust, caused by Puccinia graminis f. sp. tritici, can cause severe yield losses on susceptible wheat varieties and cultivars. Although stem rust can be controlled by the use of genetic resistance, population dynamics of P. graminis f. sp. tritici can frequently lead to defeat of wheat stem rust resistance genes. P. graminis f. sp. tritici race TKTTF caused a severe epidemic in Ethiopia on Ug99-resistant 'Digalu' in 2013 and 2014. The gene Sr11 confers resistance to race TKTTF and is present in 'Gabo 56'. We identified seven single-nucleotide polymorphism (SNP) markers linked to Sr11 from a cross between Gabo 56 and 'Chinese Spring' exploiting a 90K Infinium iSelect Custom beadchip. Five SNP markers were validated on a 'Berkut'/'Scalavatis' population that segregated for Sr11, using KBioscience competitive allele-specific polymerase chain reaction (KASP) assays. Two of the SNP markers, KASP-6BL-IWB10724 and KASP-6BL-IWB72471, were predictive of Sr11 among wheat genetic stocks, cultivars, and breeding lines from North America, Ethiopia, and Pakistan. These markers can be utilized to select for Sr11 in wheat breeding and to detect the presence of Sr11 in uncharacterized germplasm.

Original languageEnglish (US)
Pages (from-to)1352-1358
Number of pages7
JournalPhytopathology
Volume106
Issue number11
DOIs
StatePublished - 2016

Bibliographical note

Publisher Copyright:
© 2016, American Phytopathological Society. All rights reserved.

PubMed: MeSH publication types

  • Journal Article
  • Research Support, U.S. Gov't, Non-P.H.S.

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