Mass spectrometry-based proteomics: Basic principles and emerging technologies and directions

Susan K. Van Riper, Ebbing P. De Jong, John V. Carlis, Timothy J. Griffin

Research output: Chapter in Book/Report/Conference proceedingChapter

21 Scopus citations

Abstract

As the main catalytic and structural molecules within living systems, proteins are the most likely biomolecules to be affected by radiation exposure. Proteomics, the comprehensive characterization of proteins within complex biological samples, is therefore a research approach ideally suited to assess the effects of radiation exposure on cells and tissues. For comprehensive characterization of proteomes, an analytical platform capable of quantifying protein abundance, identifying post-translation modifications and revealing members of protein complexes on a systemwide level is necessary. Mass spectrometry (MS), coupled with technologies for sample fractionation and automated data analysis, provides such a versatile and powerful platform. In this chapter we offer a view on the current state of MS-proteomics, and focus on emerging technologies within three areas: (1) New instrumental methods; (2) New computational methods for peptide identification; and (3) Label-free quantification. These emerging technologies should be valuable for researchers seeking to better understand biological effects of radiation on living systems.

Original languageEnglish (US)
Title of host publicationRadiation Proteomics
Subtitle of host publicationThe Effects of Ionizing and Non-Ionizing Radiation on Cells and Tissues
PublisherSpringer New York LLC
Pages1-35
Number of pages35
ISBN (Print)9789400758957
DOIs
StatePublished - 2013

Publication series

NameAdvances in Experimental Medicine and Biology
Volume990
ISSN (Print)0065-2598

Keywords

  • 2-dimensional gel electrophoresis
  • ESI
  • Electrospray ionization
  • Glycosylation
  • Isotope labeling
  • MALDI
  • Mascot
  • Mass spectrometry
  • Matrix-assisted laser desorption/ionization
  • NanoLC
  • Nanoscale reversed-phase liquid chromatography
  • Peptide identification
  • Peptide sequencing
  • Phosphorylation
  • Proteome
  • SEQUEST
  • Sequence database

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