Mathematical model with spatially uniform regulation explains long-range bidirectional transport of early endosomes in fungal hyphae

Jia Gou, Leah Edelstein-Keshet, Jun Allard

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

In many cellular contexts, cargo is transported bidirectionally along microtubule bundles by dynein and kinesin-family motors. Upstream factors influence how individual cargoes are locally regulated, as well as how long-range transport is regulated at the whole-cell scale. Although the details of local, single-cargo bidirectional switching have been extensively studied, it remains to be elucidated how this results in cell-scale spatial organization. Here we develop a mathematical model of early endosome transport in Ustilago maydis. We demonstrate that spatiotemporally uniform regulation, with constant transition rates, results in cargo dynamics that is consistent with experimental data, including data from motor mutants. We find that microtubule arrays can be symmetric in plus-end distribution but asymmetric in binding-site distribution in a manner that affects cargo dynamics and that cargo can travel past microtubule ends in microtubule bundles. Our model makes several testable predictions, including secondary features of dynein and cargo distributions.

Original languageEnglish (US)
Pages (from-to)2408-2415
Number of pages8
JournalMolecular biology of the cell
Volume25
Issue number16
DOIs
StatePublished - 2014

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