Maximal stimulation of meiotic recombination by a yeast transcription factor requires the transcription activation domain and a DNA-binding domain

David T. Kirkpatrick, Qingqing Fan, Thomas D. Petes

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

The DNA sequences located upstream of the yeast HIS4 represent a very strong meiotic recombination hotspot. Although the activity of this hotspot requires the transcription activator Rap1p, the level of HIS4 transcription is not directly related to the level of recombination. We find that the recombination-stimulating activity of Rap1p requires the transcription activation domain of the protein. We show that a hybrid protein with the Gal4p DNA-binding domain and the Rap1p activation domain can stimulate recombination in a strain in which Gal4p-binding sites are inserted upstream of HIS4. In addition, we find recombination hotspot activity associated with the Gal4p DNA-binding sites that is independent of known transcription factors. We suggest that yeast cells have two types of recombination hotspots, α (transcription factor dependent) and β (transcription factor independent).

Original languageEnglish (US)
Pages (from-to)101-115
Number of pages15
JournalGenetics
Volume152
Issue number1
StatePublished - May 1999

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