MCM10: One tool for all-Integrity, maintenance and damage control

Research output: Contribution to journalReview articlepeer-review

30 Scopus citations

Abstract

Minichromsome maintenance protein 10 (Mcm10) is an essential replication factor that is required for the activation of the Cdc45:Mcm2-7:GINS helicase. Mcm10's ability to bind both ds and ssDNA appears vital for this function. In addition, Mcm10 interacts with multiple players at the replication fork, including DNA polymerase-α and proliferating cell nuclear antigen with which it cooperates during DNA elongation. Mcm10 lacks enzymatic function, but instead provides the replication apparatus with an oligomeric scaffold that likely acts in the coordination of DNA unwinding and DNA synthesis. Not surprisingly, loss of Mcm10 engages checkpoint, DNA repair and SUMO-dependent rescue pathways that collectively counteract replication stress and chromosome breakage. Here, we review Mcm10's structure and function and explain how it contributes to the maintenance of genome integrity.

Original languageEnglish (US)
Pages (from-to)121-130
Number of pages10
JournalSeminars in Cell and Developmental Biology
Volume30
DOIs
StatePublished - Jun 2014

Bibliographical note

Funding Information:
The authors wish to acknowledge funding from the NIH ( GM074917 ) to A-K.B. and thank Drs. Brandt Eichman and Nadine Shaban for advice regarding the crystal structures.

Keywords

  • Cancer mutations
  • DNA replication
  • Genome integrity
  • Mcm10
  • OB-fold
  • STUbL

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