Measurement of deubiquitinating enzyme activity via a suicidal HA-Ub-VS probe

Research output: Chapter in Book/Report/Conference proceedingChapter


Deubiquitinating enzymes (DUBs) are a novel "drug-able" target for cervical cancer, and small-molecule inhibitors of DUBs are currently being evaluated as novel chemotherapeutic agents. In this chapter, we describe an enzyme activity assay to assess the selectivity of a putative small-molecule DUB inhibitor toward a subset of DUBs in cancer cell lines.

Original languageEnglish (US)
Title of host publicationCervical Cancer
Subtitle of host publicationMethods and Protocols
PublisherSpringer New York
Number of pages8
ISBN (Electronic)9781493920136
ISBN (Print)9781493920129
StatePublished - Oct 27 2014


  • Cervical cancer
  • Cysteine proteases
  • Deubiquitinating enzymes
  • Dub
  • Ha-ub-vs probe
  • Metalloproteases
  • P53
  • Protease inhibitors
  • Proteases
  • Proteasome
  • Ubiquitin


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