Measurement of deubiquitinating enzyme activity via a suicidal HA-Ub-VS probe

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Deubiquitinating enzymes (DUBs) are a novel "drug-able" target for cervical cancer, and small-molecule inhibitors of DUBs are currently being evaluated as novel chemotherapeutic agents. In this chapter, we describe an enzyme activity assay to assess the selectivity of a putative small-molecule DUB inhibitor toward a subset of DUBs in cancer cell lines.

Original languageEnglish (US)
Title of host publicationCervical Cancer
Subtitle of host publicationMethods and Protocols
PublisherSpringer New York
Pages193-200
Number of pages8
ISBN (Electronic)9781493920136
ISBN (Print)9781493920129
DOIs
StatePublished - Oct 27 2014

Keywords

  • Cervical cancer
  • Cysteine proteases
  • Deubiquitinating enzymes
  • Dub
  • Ha-ub-vs probe
  • Metalloproteases
  • P53
  • Protease inhibitors
  • Proteases
  • Proteasome
  • Ubiquitin

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