Measurement of entropic unfolding force of confined DNA in the odijk regime

Jeffrey G. Reifenberger, Kevin D. Dorfman, Han Cao

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present a theoretical model and experimental data on the entropic unfolding force within DNA confined in nanochannels. We implemented a high throughput device that contains an array of 12,860 nanochannels with a 45nm cross-section capable of loading long DNA molecules (> 50 kbp) through electrophoresis. Upon insertion into the nanochannels, approximately half of the DNA molecules contain a short-lived, small fold on the leading edge that unfolds through entropic forces. Understanding the polymer physics of this system has implications for genomic mapping, as the small folds within DNA could appear as false mutation events when compared to the reference.

Original languageEnglish (US)
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PublisherChemical and Biological Microsystems Society
Pages1089-1090
Number of pages2
ISBN (Electronic)9780979806490
StatePublished - 2016
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
Duration: Oct 9 2016Oct 13 2016

Publication series

Name20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Other

Other20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
CountryIreland
CityDublin
Period10/9/1610/13/16

Bibliographical note

Funding Information:
This work was funded by the NIH, grant R01-HG006851.

Keywords

  • DNA
  • Folds
  • Nanochannel
  • Odijk regime

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