Measurement of fecal sulfide using gas chromatography and a sulfur chemiluminescence detector

Julie K. Furne; John Springfield; Thomas Koenig; Fabrizis Suarez; Michael D. Levitt

doi:10.1016/S0378-4347(00)00589-2

Measurement of fecal sulfide using gas chromatography and a sulfur chemiluminescence detector

Julie K. Furne, John Springfield, Thomas Koenig, Fabrizis Suarez, Michael D. Levitt

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12 Scopus citations

Abstract

We describe a simple technique to measure sulfide in fecal homogenates (or any other liquid milieu), which involves acidification followed by the G.C. measurement of H₂S in a gas space equilibrated with a small quantity of homogenate. An internal standard of Zn³⁵S added to the homogenate permits correction for incomplete recovery of H₂S in the gas space. The use of a sulfur chemiluminescence detector, which specifically and sensitively responds to sulfur-containing compounds, greatly facilitates this measurement.

Original language	English (US)
Pages (from-to)	253-258
Number of pages	6
Journal	Journal of Chromatography B: Biomedical Sciences and Applications
Volume	754
Issue number	1
DOIs	https://doi.org/10.1016/S0378-4347(00)00589-2
State	Published - Apr 15 2001
Externally published	Yes

Bibliographical note

Funding Information:
Supported in part by General Medical Research funds from the U.S. Department of Veterans Affairs and The National Institute of Diabetes and Digestive and Kidney Diseases grant R01 DK 13309-25.

Keywords

Sulfide

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abstract = "We describe a simple technique to measure sulfide in fecal homogenates (or any other liquid milieu), which involves acidification followed by the G.C. measurement of H2S in a gas space equilibrated with a small quantity of homogenate. An internal standard of Zn35S added to the homogenate permits correction for incomplete recovery of H2S in the gas space. The use of a sulfur chemiluminescence detector, which specifically and sensitively responds to sulfur-containing compounds, greatly facilitates this measurement.",

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AU - Springfield, John

AU - Koenig, Thomas

AU - Suarez, Fabrizis

AU - Levitt, Michael D.

N1 - Funding Information: Supported in part by General Medical Research funds from the U.S. Department of Veterans Affairs and The National Institute of Diabetes and Digestive and Kidney Diseases grant R01 DK 13309-25.

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N2 - We describe a simple technique to measure sulfide in fecal homogenates (or any other liquid milieu), which involves acidification followed by the G.C. measurement of H2S in a gas space equilibrated with a small quantity of homogenate. An internal standard of Zn35S added to the homogenate permits correction for incomplete recovery of H2S in the gas space. The use of a sulfur chemiluminescence detector, which specifically and sensitively responds to sulfur-containing compounds, greatly facilitates this measurement.

AB - We describe a simple technique to measure sulfide in fecal homogenates (or any other liquid milieu), which involves acidification followed by the G.C. measurement of H2S in a gas space equilibrated with a small quantity of homogenate. An internal standard of Zn35S added to the homogenate permits correction for incomplete recovery of H2S in the gas space. The use of a sulfur chemiluminescence detector, which specifically and sensitively responds to sulfur-containing compounds, greatly facilitates this measurement.

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Measurement of fecal sulfide using gas chromatography and a sulfur chemiluminescence detector

Abstract

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