Measurement of the caspase-1-like activity of vacuolar processing enzyme in plants

Noriyuki Hatsugai; Ikuko Hara-Nishimura

doi:10.1007/978-1-4939-7668-3_15

Measurement of the caspase-1-like activity of vacuolar processing enzyme in plants

Noriyuki Hatsugai, Ikuko Hara-Nishimura

Plant and Microbial Biology

Research output: Chapter in Book/Report/Conference proceeding › Chapter

1 Scopus citations

Abstract

Caspase-like activities are essential to regulate programed cell death in plants. Although no caspase orthologous enzymes with aspartic acid specificity have been identified in plants, vacuolar processing enzyme (VPE) exhibits a caspase-1-like activity. In this chapter, we introduce two methods for the measurement of the caspase-1-like/VPE activity. These methods are based on the cleavage of caspase-1 specific synthetic substrates and on monitoring the active forms of VPE using a biotinylated-inhibitor blot analysis. Both methods are also adaptable to other plant caspase-like activities.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	163-171
Number of pages	9
DOIs	https://doi.org/10.1007/978-1-4939-7668-3_15
State	Published - 2018

Publication series

Name	Methods in Molecular Biology
Volume	1743
ISSN (Print)	1064-3745

Bibliographical note

Funding Information:
This work was supported by Grants-in-Aid for Scientific Research (no. 15H05776) to I.H-.N. from Japan Society for the Promotion of Science (JSPS).

Publisher Copyright:
© 2018, Springer Science+Business Media, LLC.

Keywords

Caspase-1-like activity
Plants
VPE
Vacuolar processing enzyme

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abstract = "Caspase-like activities are essential to regulate programed cell death in plants. Although no caspase orthologous enzymes with aspartic acid specificity have been identified in plants, vacuolar processing enzyme (VPE) exhibits a caspase-1-like activity. In this chapter, we introduce two methods for the measurement of the caspase-1-like/VPE activity. These methods are based on the cleavage of caspase-1 specific synthetic substrates and on monitoring the active forms of VPE using a biotinylated-inhibitor blot analysis. Both methods are also adaptable to other plant caspase-like activities.",

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AB - Caspase-like activities are essential to regulate programed cell death in plants. Although no caspase orthologous enzymes with aspartic acid specificity have been identified in plants, vacuolar processing enzyme (VPE) exhibits a caspase-1-like activity. In this chapter, we introduce two methods for the measurement of the caspase-1-like/VPE activity. These methods are based on the cleavage of caspase-1 specific synthetic substrates and on monitoring the active forms of VPE using a biotinylated-inhibitor blot analysis. Both methods are also adaptable to other plant caspase-like activities.

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Measurement of the caspase-1-like activity of vacuolar processing enzyme in plants

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