Measuring bacterial adaptation dynamics at single-cell level using a microfluidic chemostat and time-lapse fluorescence microscopy

Zhicheng Long, Anne Olliver, Elisa Brambilla, Bianca Sclavi, Marco Cosentino Lagomarsino, Kevin D. Dorfman

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We report the operation of a microchemostat device for monitoring the dynamic response of single E. coli bacterial cells to nutritional downshift using time-lapse fluorescence microscopy. We observed two E. coli strains containing different promoter-reporter gene constructs. Both strains have similar adaptation dynamics for cell size rearrangement. However, the GFP expression dynamics of these two strains are distinctly different. The mean GFP concentration in the P1 strain cells with a ribosomal RNA promoter changes rapidly during nutritional downshift, while that in the P5 strain cells with a constitutive promoter remains relatively stable.

Original languageEnglish (US)
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages834-836
Number of pages3
ISBN (Electronic)9780979806476
StatePublished - Jan 1 2014
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: Oct 26 2014Oct 30 2014

Publication series

Name18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

Other

Other18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
CountryUnited States
CitySan Antonio
Period10/26/1410/30/14

Keywords

  • Bacterial adaptation
  • Microchemostat
  • Single-cell dynamics

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