Measuring protein self-diffusion in protein-protein mixtures using a pulsed gradient spin-echo technique with WATERGATE and isotope filtering

Irina V. Nesmelova, Djaudat S Idiyatullin, Kevin H Mayo

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Here we report a modified pulsed gradient spin-echo (PGSTE) pulse sequence to measure diffusion coefficients. This approach incorporates WATERGATE combined with isotopic filtering into a standard PGSTE experiment. Doing this eliminates much of the disadvantages from the combination of diffusion encoding and heteronuclear selection intervals and allows for facile modification of the diffusion pulse sequence with flexibility of the time period between RF pulses. The new diffusion pulse sequence is demonstrated using an 15N-labeled peptide and an 15N-labeled protein in a mixture with a protein of similar size.

Original languageEnglish (US)
Pages (from-to)129-133
Number of pages5
JournalJournal of Magnetic Resonance
Volume166
Issue number1
DOIs
StatePublished - Jan 1 2004

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