TY - JOUR
T1 - Mechanism and role of growth arrest in programmed (apoptotic) death of prostatic cancer cells induced by thapsigargin
AU - Lin, Xiaohui S.
AU - Denmeade, Samuel R.
AU - Cisek, Lars
AU - Isaacs, John T.
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 1997/11/1
Y1 - 1997/11/1
N2 - BACKGROUND. More than 95% of metastatic androgen independent prostatic cancer cells per day are in a proliferatively quiescent G(o) state [Berges et al.: Clin Cancer Res 1:473-480, 1995] limiting their responsiveness to anti- proliferative chemotherapeutic agents. Novel therapeutics capable of activating the programmed (apoptotic) death pathway in these cells without requiring entrance into the proliferative cell cycle are urgently needed. Thapsigargin (TG) treatment of rapidly growing androgen independent prostatic cancer cells arrests such cells in G(o) and induces their programmed death. This raises not only the issue of the mechanism for such growth arrest, but also whether this programmed death is simply a response of rapidly growing cells to growth arrest making cytotoxicity still dependent upon the initial rate of cell proliferation. METHODS. To resolve the mechanism of TG induced growth arrest, rat AT3.1 prostatic cancer cells were analyzed for RNA and protein expression of the growth arrest gene, gadd153, intracellular free Ca2+ levels (Ca(j)), and cell cycle distribution on exposure to TG alone and in combination with Ca2+ chelation induced by BAPTA-AM or BAPTA- AM/EGTA. To resolve whether growth arrest is required for TG cytotoxicity, primary cultures of proliferatively quiescent, human prostatic cancer cells were exposed to TG. RESULTS. Co-treatment of androgen independent AT-3 rat prostatic cancer cells with the Ca(i) chelator BAPTA plus TG prevented growth arrest, as monitored by DNA flow cytometry, and failure to induce mRNA and protein for gadd153, demonstrating that growth arrest is due to Ca(i) elevation, not depletion of intracellular Ca2+ pools. In addition, proliferatively quiescent G(o) primary cultures of human prostatic cancer cells were resistant to anti-proliferative agents, but could be induced to undergo programmed death by TG as documented by morphological criteria and 14C-labeled DNA fragmentation assays. CONCLUSIONS. These results demonstrate that TG with its ability to elevate Ca(i) induces proliferating prostate cancer cells to growth arrest. Such Ca(i) dependent growth arrest is not required, however, since TG can induce the programmed death of proliferatively quiescent G(o) prostatic cancer cells without requiring either growth arrest or progression through the proliferative cell cycle.
AB - BACKGROUND. More than 95% of metastatic androgen independent prostatic cancer cells per day are in a proliferatively quiescent G(o) state [Berges et al.: Clin Cancer Res 1:473-480, 1995] limiting their responsiveness to anti- proliferative chemotherapeutic agents. Novel therapeutics capable of activating the programmed (apoptotic) death pathway in these cells without requiring entrance into the proliferative cell cycle are urgently needed. Thapsigargin (TG) treatment of rapidly growing androgen independent prostatic cancer cells arrests such cells in G(o) and induces their programmed death. This raises not only the issue of the mechanism for such growth arrest, but also whether this programmed death is simply a response of rapidly growing cells to growth arrest making cytotoxicity still dependent upon the initial rate of cell proliferation. METHODS. To resolve the mechanism of TG induced growth arrest, rat AT3.1 prostatic cancer cells were analyzed for RNA and protein expression of the growth arrest gene, gadd153, intracellular free Ca2+ levels (Ca(j)), and cell cycle distribution on exposure to TG alone and in combination with Ca2+ chelation induced by BAPTA-AM or BAPTA- AM/EGTA. To resolve whether growth arrest is required for TG cytotoxicity, primary cultures of proliferatively quiescent, human prostatic cancer cells were exposed to TG. RESULTS. Co-treatment of androgen independent AT-3 rat prostatic cancer cells with the Ca(i) chelator BAPTA plus TG prevented growth arrest, as monitored by DNA flow cytometry, and failure to induce mRNA and protein for gadd153, demonstrating that growth arrest is due to Ca(i) elevation, not depletion of intracellular Ca2+ pools. In addition, proliferatively quiescent G(o) primary cultures of human prostatic cancer cells were resistant to anti-proliferative agents, but could be induced to undergo programmed death by TG as documented by morphological criteria and 14C-labeled DNA fragmentation assays. CONCLUSIONS. These results demonstrate that TG with its ability to elevate Ca(i) induces proliferating prostate cancer cells to growth arrest. Such Ca(i) dependent growth arrest is not required, however, since TG can induce the programmed death of proliferatively quiescent G(o) prostatic cancer cells without requiring either growth arrest or progression through the proliferative cell cycle.
KW - Apoptosis
KW - Calcium
KW - Gadd153
KW - Growth arrest
KW - Programmed cell death
KW - Thapsigargin
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U2 - 10.1002/(SICI)1097-0045(19971101)33:3<201::AID-PROS9>3.0.CO;2-L
DO - 10.1002/(SICI)1097-0045(19971101)33:3<201::AID-PROS9>3.0.CO;2-L
M3 - Article
C2 - 9365549
AN - SCOPUS:0030690164
SN - 0270-4137
VL - 33
SP - 201
EP - 207
JO - Prostate
JF - Prostate
IS - 3
ER -