Membrane Active Peptides Remove Surface Adsorbed Protein Corona From Extracellular Vesicles of Red Blood Cells

Priyanka Singh, Imola Cs Szigyártó, Maria Ricci, Ferenc Zsila, Tünde Juhász, Judith Mihály, Szilvia Bősze, Éva Bulyáki, József Kardos, Diána Kitka, Zoltán Varga, Tamás Beke-Somfai

Research output: Contribution to journalArticlepeer-review

Abstract

Besides the outstanding potential in biomedical applications, extracellular vesicles (EVs) are also promising candidates to expand our knowledge on interactions between vesicular surface proteins and small-molecules which exert biomembrane-related functions. Here we provide mechanistic details on interactions between membrane active peptides with antimicrobial effect (MAPs) and red blood cell derived EVs (REVs) and we demonstrate that they have the capacity to remove members of the protein corona from REVs even at lower than 5 μM concentrations. In case of REVs, the Soret-band arising from the membrane associated hemoglobins allowed to follow the detachment process by flow-Linear Dichroism (flow-LD). Further on, the significant change on the vesicle surfaces was confirmed by transmission electron microscopy (TEM). Since membrane active peptides, such as melittin have the affinity to disrupt vesicles, a combination of techniques, fluorescent antibody labeling, microfluidic resistive pulse sensing, and flow-LD were employed to distinguish between membrane destruction and surface protein detachment. The removal of protein corona members is a newly identified role for the investigated peptides, which indicates complexity of their in vivo function, but may also be exploited in synthetic and natural nanoparticle engineering. Furthermore, results also promote that EVs can be used as improved model systems for biophysical studies providing insight to areas with so far limited knowledge.

Original languageEnglish (US)
Article number703
JournalFrontiers in Chemistry
Volume8
DOIs
StatePublished - Aug 11 2020
Externally publishedYes

Bibliographical note

Funding Information:
The authors gratefully acknowledge Ter?z Kiss for assisting FF-TEM measurements. Funding. This work was funded by the Momentum Programme [LP2016-2 (TB-S)] of the Hungarian Academy of Sciences, by the National Competitiveness and Excellence Program (NVKP_16-1-2016-0007), of the National Research, Development and Innovation Office, NKFIH, Hungary and the GINOP grant (BIONANO_GINOP-2.3.2-15-2016-00017). ZV was supported by the J?nos Bolyai Research Fellowship and from the National Research, Development and Innovation Office under grant number PD121326. This work was completed in the ELTE Thematic Excellence Programme (Szint+) supported by the Hungarian Ministry for Innovation and Technology.

Funding Information:
This work was funded by the Momentum Programme [LP2016-2 (TB-S)] of the Hungarian Academy of Sciences, by the National Competitiveness and Excellence Program (NVKP_16-1-2016-0007), of the National Research, Development and Innovation Office, NKFIH, Hungary and the GINOP grant (BIONANO_GINOP-2.3.2-15-2016-00017). ZV was supported by the János Bolyai Research Fellowship and from the National Research, Development and Innovation Office under grant number PD121326. This work was completed in the ELTE Thematic Excellence Programme (Szint+) supported by the Hungarian Ministry for Innovation and Technology.

Publisher Copyright:
© Copyright © 2020 Singh, Szigyártó, Ricci, Zsila, Juhász, Mihály, Bősze, Bulyáki, Kardos, Kitka, Varga and Beke-Somfai.

Keywords

  • antimicrobial peptide
  • biomembrane
  • extracellular vesicles
  • liposome
  • protein corona

PubMed: MeSH publication types

  • Journal Article

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