Membrane-bound guaiacol peroxidases from maize (Zea mays L.) roots are regulated by methyl jasmonate, salicylic acid, and pathogen elicitors

Angela Mika, Marike Johanne Boenisch, David Hopff, Sabine Lüthje

Research output: Contribution to journalArticlepeer-review

59 Scopus citations

Abstract

Plant peroxidases are involved in numerous cellular processes in plant development and stress responses. Four plasma membrane-bound peroxidases have been identified and characterized in maize (Zea mays L.) roots. In the present study, maize seedlings were treated with different stresses and signal compounds, and a functional analysis of these membrane-bound class III peroxidases (pmPOX1, pmPOX2a, pmPOX2b, and pmPOX3) was carried out. Total guaiacol peroxidase activities from soluble and microsomal fractions of maize roots were compared and showed weak changes. By contrast, total plasma membrane and washed plasma membrane peroxidase activities, representing peripheral and integral membrane proteins, revealed strong changes after all of the stresses applied. A proteomic approach using 2D-PAGE analysis showed that pmPOX3 was the most abundant class III peroxidase at plasma membranes of control plants, followed by pmPOX2a >pmPOX2b >pmPOX1. The molecular mass (63 kDa) and the isoelectric point (9.5) of the pmPOX2a monomer were identified for the first time. The protein levels of all four enzymes changed in response to multiple stresses. While pmPOX2b was the only membrane peroxidase down-regulated by wounding, all four enzymes were differentially but strongly stimulated by methyl jasmonate, salicylic acid, and elicitors (Fusarium graminearum and Fusarium culmorum extracts, and chitosan) indicating their function in pathogen defence. Oxidative stress applied as H2O2 treatment up-regulated pmPOX2b >pmPOX2a, while pmPOX3 was down-regulated. Treatment with the phosphatase inhibitor chantharidin resulted in distinct responses.

Original languageEnglish (US)
Pages (from-to)831-841
Number of pages11
JournalJournal of experimental botany
Volume61
Issue number3
DOIs
StatePublished - Mar 2010

Bibliographical note

Funding Information:
The authors wish to thank Aslihan Ayar, Janis Hantke, and Natalie Cyranck, for excellent technical assistance. This work was supported by the Deutsche Forschungsgemein-schaft (DFG Lu-668/4-2) and by the University of Hamburg (PhD student’s grant no. HmbNFG to DH).

Keywords

  • Class III peroxidases
  • Methyl jasmonate
  • Oxidative stress
  • Plasma membrane-bound
  • Salicylic acid
  • Zea mays L

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