Metabolic response of porcine colon explants to in vitro infection by Brachyspira hyodysenteriae: a leap into disease pathophysiology

Thijs Welle, Anna T. Hoekstra, Ineke A.J.J.M. Daemen, Celia R. Berkers, Matheus O. Costa

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Introduction: Swine dysentery caused by Brachyspira hyodysenteriae is a production limiting disease in pig farming. Currently antimicrobial therapy is the only treatment and control method available. Objective: The aim of this study was to characterize the metabolic response of porcine colon explants to infection by B. hyodysenteriae. Methods: Porcine colon explants exposed to B. hyodysenteriae were analyzed for histopathological, metabolic and pro-inflammatory gene expression changes. Results: Significant epithelial necrosis, increased levels of l-citrulline and IL-1α were observed on explants infected with B. hyodysenteriae. Conclusions: The spirochete induces necrosis in vitro likely through an inflammatory process mediated by IL-1α and NO.

Original languageEnglish (US)
Article number83
JournalMetabolomics
Volume13
Issue number7
DOIs
StatePublished - Jul 1 2017

Bibliographical note

Funding Information:
This work was funded by an Utrecht University, Department of Farm Animal Health internal research fund (MC) and a NWO Gravitation project Institute for Chemical Immunology (CB).

Keywords

  • Brachyspira
  • Host-pathogen interactions
  • In vitro organ culture
  • Nitric oxide
  • Pathophysiology
  • Swine dysentery

Fingerprint Dive into the research topics of 'Metabolic response of porcine colon explants to in vitro infection by Brachyspira hyodysenteriae: a leap into disease pathophysiology'. Together they form a unique fingerprint.

Cite this