Method of peptide nucleic acid (PNA)-mediated antisense inhibition of gene expression in Campylobacter jejuni

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Peptide nucleic acid (PNA) is an oligonucleotide mimic that recognizes and binds to nucleic acids. The strong binding affinity of PNA to mRNA coupled with its high sequence specificity enable antisense PNA to selectively inhibit (i.e., knockdown) the protein synthesis of a target gene. This novel technology provides a powerful tool for Campylobacter studies because molecular techniques have been relatively less well-developed for this bacterium as compared to other pathogens, such as Escherichia coli and Salmonella. This chapter describes a protocol for PNA-mediated antisense inhibition of gene expression in Campylobacter jejuni .

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages43-49
Number of pages7
DOIs
StatePublished - Jan 1 2017
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1512
ISSN (Print)1064-3745

Keywords

  • Antisense
  • Campylobacter
  • Gene knockdown
  • Peptide nucleic acid (PNA)

Fingerprint Dive into the research topics of 'Method of peptide nucleic acid (PNA)-mediated antisense inhibition of gene expression in Campylobacter jejuni'. Together they form a unique fingerprint.

Cite this