Through a simple chemical activation of biomolecules present in the outer structures of microbial cells, microorganisms can be rapidly isolated on gold-coated surfaces in a microfluidic device with over 99% capture efficiency. Bacterial and fungal cells can be selectively captured, concentrated and retrieved for further analysis.
Bibliographical noteFunding Information:
This work was supported by the US National Science Foundation award No. 1605191, and by the Minnesota Environment and Natural Resource Trust Fund as recommended by the Legislative Citizen Commission on Minnesota’s Resources, through the Minnesota Invasive Terrestrial Plants and Pests Center. Support was also provided by University of Minnesota MnDRIVE Global Food Venture, the USDA National Institute of Food and Agriculture Hatch project 1006789, and the Schwan Food Company Fellowship. We would like to thank Dr Jennifer Juzwik for providing the culture of the C. fagacearum fungus.