Using conventional triple-resonance nuclear magnetic resonance (NMR) experiments with a 1 mm triple-resonance microcoil NMR probe, we determined near complete resonance assignments and three-dimensional (3D) structure of the 68-residue Methanosarcina mazei TRAM protein using only 72 μg (6 μl, 1.4 mM) of protein. This first example of a complete solution NMR structure determined using microgram quantities of protein demonstrates the utility of microcoil-probe NMR technologies for protein samples that can be produced in only limited quantities.
Bibliographical noteFunding Information:
We thank T. Acton for helpful discussions. This work was supported by a grant from the Protein Structure Initiative of the US National Institutes of Health (U54 GM074958).