Microgram-scale protein structure determination by NMR

James M. Aramini; Paolo Rossi; Clemens Anklin; Rong Xiao; Gaetano T. Montelione

doi:10.1038/nmeth1051

Microgram-scale protein structure determination by NMR

James M. Aramini, Paolo Rossi, Clemens Anklin, Rong Xiao, Gaetano T. Montelione

Biochemistry, Molecular Biology, and Biophysics (CBS)

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

Using conventional triple-resonance nuclear magnetic resonance (NMR) experiments with a 1 mm triple-resonance microcoil NMR probe, we determined near complete resonance assignments and three-dimensional (3D) structure of the 68-residue Methanosarcina mazei TRAM protein using only 72 μg (6 μl, 1.4 mM) of protein. This first example of a complete solution NMR structure determined using microgram quantities of protein demonstrates the utility of microcoil-probe NMR technologies for protein samples that can be produced in only limited quantities.

Original language	English (US)
Pages (from-to)	491-493
Number of pages	3
Journal	Nature Methods
Volume	4
Issue number	6
DOIs	https://doi.org/10.1038/nmeth1051
State	Published - Jun 2007

Bibliographical note

Funding Information:
We thank T. Acton for helpful discussions. This work was supported by a grant from the Protein Structure Initiative of the US National Institutes of Health (U54 GM074958).

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Microgram-scale protein structure determination by NMR

Abstract

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