Microsatellite markers for the hybridizing tiger swallowtails, Papilio glaucus and P. canadensis, and their applicability to historic specimens and congeneric species

Carsten Nowak, Christian M. Brown, Jessica J. Hellmann

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

We report the development of microsatellite markers for the sister species Papilio glaucus and P. canadensis (Papilionidae: Lepidoptera). All 16 markers displayed a high degree of variation in both species, ranging from eight to 24 alleles per locus. Substantial heterozygote deficits were observed for several loci, indicating the presence of null alleles. All markers were successfully used to genotype dried samples from a historical collection. Cross-species amplification with six additional Papilio species showed that most loci can be used to study genetic variation in other closely related species of tiger swallowtails.

Original languageEnglish (US)
Pages (from-to)800-803
Number of pages4
JournalMolecular Ecology Resources
Volume9
Issue number3
DOIs
StatePublished - May 2009
Externally publishedYes

Keywords

  • Canadian tiger swallowtail
  • Eastern tiger swallowtail
  • Genetic markers
  • Hybridization
  • Lepidoptera
  • Sister species

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