A rapid minicolumn technique is described that has been used for the isolation and mass spectral quantification of indole-3-acetic acid from cherry, a difficult to analyze tissue. The method used the resin XAD-7 to trap impurities. Samples were further purified on an amino minicolumn and then by high-performance liquid chromatography on a C18 column. After methylation, the samples were analyzed by selected ion monitoring gas chromatography-mass spectrometry. 13C6indole-3-acetic acid was employed as an internal standard for isotope dilution calculations.
Bibliographical noteFunding Information:
l Part of this work was carried out under cooperative agreement No. 56-32U4-7-001 (RSA-7-001-010) of the USDA/ARS and the University of Maryland and, in addition, this work was funded by United States National Science Foundation Metabolic Biology grant DMB-8617171. ** Current address: Department of Pharmacology and Molecular Science, Johns Hopkins University, Baltimore, MD 21205, U.S.A.