Microscale isolation technique for quantitative gas chromatography-mass spectrometry analysis of indole-3-acetic acid from cherry (prunus cerasus l.)

Rita Baraldi, Kai Hsien Chen, Jerry D. Cohen

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8 Scopus citations

Abstract

A rapid minicolumn technique is described that has been used for the isolation and mass spectral quantification of indole-3-acetic acid from cherry, a difficult to analyze tissue. The method used the resin XAD-7 to trap impurities. Samples were further purified on an amino minicolumn and then by high-performance liquid chromatography on a C18 column. After methylation, the samples were analyzed by selected ion monitoring gas chromatography-mass spectrometry. 13C6indole-3-acetic acid was employed as an internal standard for isotope dilution calculations.

Original languageEnglish (US)
Pages (from-to)301-306
Number of pages6
JournalJournal of Chromatography A
Volume442
Issue numberC
DOIs
StatePublished - 1988

Bibliographical note

Funding Information:
l Part of this work was carried out under cooperative agreement No. 56-32U4-7-001 (RSA-7-001-010) of the USDA/ARS and the University of Maryland and, in addition, this work was funded by United States National Science Foundation Metabolic Biology grant DMB-8617171. ** Current address: Department of Pharmacology and Molecular Science, Johns Hopkins University, Baltimore, MD 21205, U.S.A.

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