Microscopic analysis of fluorescence resonance energy transfer (FRET).

Brian Herman, R. Venkata Krishnan, Victoria E. Centonze

Research output: Contribution to journalReview articlepeer-review

28 Scopus citations

Abstract

We describe here detailed practical procedures for implementing various approaches of fluorescence resonance energy transfer (FRET) in microscope-based measurements. A comprehensive theoretical formalism is developed and the different experimental procedures are outlined. A step-by-step protocol is provided for preparing the specimens for FRET measurements, data acquisition procedures, analysis, and quantification. Particular emphasis is given to exemplify the FRET applications in the study of protein-protein interactions.

Original languageEnglish (US)
Pages (from-to)351-370
Number of pages20
JournalMethods in molecular biology (Clifton, N.J.)
Volume261
DOIs
StatePublished - 2004

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