Microtubule translocation caused by three subspecies of inner-arm dynein from Chlamydomonas flagella

Osamu Kagami, Saeko Takada, Ritsu Kamiya

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

To help understand the function of inner-arm dynein in nagellar motility, dynein samples from an outer arm-missing mutant of Chlamydomonas (odal) were examined for the ability to translocate microtubules in vitro. High-salt extract ofaxonemes containing inner-arm dynein was separated by ion-exchange chromatography into 7 peak fractions with ATPase activities. Of these, three fractions containing different sets of dynein heavy chains translocated microtubules. The maximal velocities were all between 3 and 5 μm s, which were comparable to the microtubule sliding rate in disintegrating oda axonemes.

Original languageEnglish (US)
Pages (from-to)179-182
Number of pages4
JournalFEBS Letters
Volume264
Issue number2
DOIs
StatePublished - May 21 1990

Keywords

  • Dynein
  • Microtubule
  • Motility
  • Translocation

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