TY - JOUR
T1 - Mild, orthogonal solid‐phase peptide synthesis
T2 - use of Nα‐dithiasuccinoyl (Dts) amino acids and N‐(iso‐propyldithio)carbonylproline, together with p‐alkoxybenzyl ester anchoring linkages
AU - ALBERICIO, FERNANDO
AU - BARANY, GEORGE
PY - 1987/8
Y1 - 1987/8
N2 - Several Nα‐dithiasuccinoyl (Dts) amino acids (1) have been esterified without race‐mization by use of either N,N'‐dicyclohexylcarbodiimide or 1‐(3‐dimethylamino‐propyl)‐3‐ethylcarbodiimide hydrochloride, each in the presence of 4‐dimethylaminopyridine (0.1 equiv.), to 2, 4, 5‐trichlorophenyl 4′‐hydroxymethylphenoxyacetate (10) or the corresponding propionate (11). The resultant handle derivatives (8,9) were purified and then quantitatively attached onto aminomethyl supports by couplings using as solvent N,N‐dimethylformamide containing 1‐hydroxybenzotriazole (0.1 M). This methodology facilitates anchoring of Dts‐amino acids as p‐alkoxybenzyl esters, which can be cleaved in good yields by trifluoroacetic acid‐dichloromethane (1:1) at 25°. Model experiments established that quantitative thiolytic removal (>99.8%) of the Dts group occurs with (i) β‐mercaptoethanol (0.5M)‐N,N‐diisopropylethylamine (0.5M) in dichloromethane, 2 times 2min; (ii) N‐methylmercaptoacetamide (0.5M)‐N‐methylmorpholine (0.5M) in dichloromethane, 2 times 2min; and (iii) N‐methylmercaptoacetamide (0.5M)‐1‐hydroxybenzotriazole (0.1M) in N,N‐dimethylformamide, 2 times 2 min. The susceptibility of the Dts functionality to nucleophiles was also defined, including demonstration of tertiary amine‐catalyzed hydantoin formation from Dts‐dipeptidyl units, but side reactions from these processes are entirely avoided under appropriate conditions relevant to peptide synthesis. These observations were exploited to devise efficient, racemization‐free solid‐phase syntheses of a number of model peptides in high yields and purities, including L‐leucyl‐L‐alanylglycyl‐L‐valine, H‐Gly6‐Val‐OH, H‐Met‐Ala‐Gly‐OH, methionine‐enkephalin, and bradykinin.
AB - Several Nα‐dithiasuccinoyl (Dts) amino acids (1) have been esterified without race‐mization by use of either N,N'‐dicyclohexylcarbodiimide or 1‐(3‐dimethylamino‐propyl)‐3‐ethylcarbodiimide hydrochloride, each in the presence of 4‐dimethylaminopyridine (0.1 equiv.), to 2, 4, 5‐trichlorophenyl 4′‐hydroxymethylphenoxyacetate (10) or the corresponding propionate (11). The resultant handle derivatives (8,9) were purified and then quantitatively attached onto aminomethyl supports by couplings using as solvent N,N‐dimethylformamide containing 1‐hydroxybenzotriazole (0.1 M). This methodology facilitates anchoring of Dts‐amino acids as p‐alkoxybenzyl esters, which can be cleaved in good yields by trifluoroacetic acid‐dichloromethane (1:1) at 25°. Model experiments established that quantitative thiolytic removal (>99.8%) of the Dts group occurs with (i) β‐mercaptoethanol (0.5M)‐N,N‐diisopropylethylamine (0.5M) in dichloromethane, 2 times 2min; (ii) N‐methylmercaptoacetamide (0.5M)‐N‐methylmorpholine (0.5M) in dichloromethane, 2 times 2min; and (iii) N‐methylmercaptoacetamide (0.5M)‐1‐hydroxybenzotriazole (0.1M) in N,N‐dimethylformamide, 2 times 2 min. The susceptibility of the Dts functionality to nucleophiles was also defined, including demonstration of tertiary amine‐catalyzed hydantoin formation from Dts‐dipeptidyl units, but side reactions from these processes are entirely avoided under appropriate conditions relevant to peptide synthesis. These observations were exploited to devise efficient, racemization‐free solid‐phase syntheses of a number of model peptides in high yields and purities, including L‐leucyl‐L‐alanylglycyl‐L‐valine, H‐Gly6‐Val‐OH, H‐Met‐Ala‐Gly‐OH, methionine‐enkephalin, and bradykinin.
KW - 4‐hydroxymethylphenoxyacetate and 3‐(4′‐hydroxymethylphenoxy)propionate handles
KW - N‐(iso‐propyldithio)carbonylproline
KW - N‐dithiasuccinoyl amino acids
KW - hydantoins
KW - orthogonal protection schemes
KW - p‐alkoxybenzyl esters
KW - solid‐phase peptide synthesis
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U2 - 10.1111/j.1399-3011.1987.tb03327.x
DO - 10.1111/j.1399-3011.1987.tb03327.x
M3 - Article
C2 - 3679669
AN - SCOPUS:0023394167
SN - 0367-8377
VL - 30
SP - 177
EP - 205
JO - International Journal of Peptide and Protein Research
JF - International Journal of Peptide and Protein Research
IS - 2
ER -