Miniaturized device for whole cortex mesoscale imaging in freely behaving mice

Mathew L. Rynes; Daniel Surinach; Micheal Laroque; Samantha Linn; Judith Dominguez; Leila Ghanbari; Suhasa B. Kodandaramaiah

doi:10.1117/12.2550395

Miniaturized device for whole cortex mesoscale imaging in freely behaving mice

Mathew L. Rynes, Daniel Surinach, Micheal Laroque, Samantha Linn, Judith Dominguez, Leila Ghanbari, Suhasa B. Kodandaramaiah

Mechanical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

The advent of genetically-encoded calcium indicators, along with surgical preparations such as thinned skulls or refractive index matched skulls, have enabled mesoscale cortical activity imaging in headfixed mice. Such imaging studies have revealed complex patterns of coordinated activity acroß the cortical surface during a variety of behaviors. However, neural activity during free, unrestrained behavior significantly differs from that observed in headfixed animals. The ability to perform mesoscale imaging of the cortex in freely behaving mice may launch new avenues of scientific enquiry. Here we present the 'Mesoscope', a miniature, head-mountable imaging device compatible with transparent polymer skulls recently developed by our group. With an 8x10 mm field of view, the Mesoscope can image most of the mouse dorsal cortex and weighs only 3.7 grams. Here, we show preliminary behavioral experiments and mesoscale imaging in anesthetized mice.

Original language	English (US)
Title of host publication	Neural Imaging and Sensing 2020
Editors	Qingming Luo, Jun Ding, Ling Fu
Publisher	SPIE
ISBN (Electronic)	9781510632158
DOIs	https://doi.org/10.1117/12.2550395
State	Published - 2020
Event	Neural Imaging and Sensing 2020 - San Francisco, United States Duration: Feb 3 2020 → Feb 5 2020

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	11226
ISSN (Print)	1605-7422

Conference

Conference	Neural Imaging and Sensing 2020
Country/Territory	United States
City	San Francisco
Period	2/3/20 → 2/5/20

Bibliographical note

Publisher Copyright:
© COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.

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Rynes, M. L., Surinach, D., Laroque, M., Linn, S., Dominguez, J., Ghanbari, L., & Kodandaramaiah, S. B. (2020). Miniaturized device for whole cortex mesoscale imaging in freely behaving mice. In Q. Luo, J. Ding, & L. Fu (Eds.), Neural Imaging and Sensing 2020 Article 112260G (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 11226). SPIE. https://doi.org/10.1117/12.2550395

Miniaturized device for whole cortex mesoscale imaging in freely behaving mice. / Rynes, Mathew L.; Surinach, Daniel; Laroque, Micheal et al.
Neural Imaging and Sensing 2020. ed. / Qingming Luo; Jun Ding; Ling Fu. SPIE, 2020. 112260G (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 11226).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Rynes, ML, Surinach, D, Laroque, M, Linn, S, Dominguez, J, Ghanbari, L & Kodandaramaiah, SB 2020, Miniaturized device for whole cortex mesoscale imaging in freely behaving mice. in Q Luo, J Ding & L Fu (eds), Neural Imaging and Sensing 2020., 112260G, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 11226, SPIE, Neural Imaging and Sensing 2020, San Francisco, United States, 2/3/20. https://doi.org/10.1117/12.2550395

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Miniaturized device for whole cortex mesoscale imaging in freely behaving mice

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