Mitochondrial DNA variants influence mitochondrial bioenergetics in Drosophila melanogaster

C. C. Correa; W. C. Aw; R. G. Melvin; N. Pichaud; J. W.O. Ballard

doi:10.1016/j.mito.2012.06.005

Mitochondrial DNA variants influence mitochondrial bioenergetics in Drosophila melanogaster

C. C. Correa, W. C. Aw, R. G. Melvin, N. Pichaud, J. W.O. Ballard

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Abstract

The influence of mitochondrial DNA (mtDNA) mutations on human disease has been extensively studied, but the impact of mutations within the adaptive range is debated. We studied males from lines of Drosophila melanogaster that have a highly standardized nuclear genome but different mtDNA, at two ages. We measured mitochondrial respiration on permeabilized muscle fibers, hydrogen peroxide production of isolated mitochondria and mtDNA copy number of whole individuals. The results show that a small set of naturally occurring mtDNA mutations can have a significant influence on mitochondrial bioenergetics that may change as the organism ages.

Original language	English (US)
Pages (from-to)	459-464
Number of pages	6
Journal	Mitochondrion
Volume	12
Issue number	4
DOIs	https://doi.org/10.1016/j.mito.2012.06.005
State	Published - Jul 2012
Externally published	Yes

Bibliographical note

Funding Information:
The work was supported by University of New South Wales internal grants and ARC grant DP110104542 . Members of the Ballard Lab and an anonymous reviewer provided useful comments on a draft of the manuscript.

Keywords

Mitochondrial bioenergetics
MtDNA copy number
Reactive oxygen species

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title = "Mitochondrial DNA variants influence mitochondrial bioenergetics in Drosophila melanogaster",

abstract = "The influence of mitochondrial DNA (mtDNA) mutations on human disease has been extensively studied, but the impact of mutations within the adaptive range is debated. We studied males from lines of Drosophila melanogaster that have a highly standardized nuclear genome but different mtDNA, at two ages. We measured mitochondrial respiration on permeabilized muscle fibers, hydrogen peroxide production of isolated mitochondria and mtDNA copy number of whole individuals. The results show that a small set of naturally occurring mtDNA mutations can have a significant influence on mitochondrial bioenergetics that may change as the organism ages.",

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AU - Melvin, R. G.

AU - Pichaud, N.

AU - Ballard, J. W.O.

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KW - Mitochondrial bioenergetics

KW - MtDNA copy number

KW - Reactive oxygen species

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Mitochondrial DNA variants influence mitochondrial bioenergetics in Drosophila melanogaster

Abstract

Bibliographical note

Keywords

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