TY - JOUR
T1 - Mitotic noncoding RNA processing promotes kinetochore and spindle assembly in Xenopus
AU - Grenfell, Andrew W.
AU - Heald, Rebecca
AU - Strzelecka, Magdalena
N1 - Publisher Copyright:
© 2016 Grenfell et al.
PY - 2016/7/18
Y1 - 2016/7/18
N2 - Transcription at the centromere of chromosomes plays an important role in kinetochore assembly in many eukaryotes, and noncoding RNAs contribute to activation of the mitotic kinase Aurora B. However, little is known about how mitotic RNA processing contributes to spindle assembly. We found that inhibition of transcription initiation or RNA splicing, but not translation, leads to spindle defects in Xenopus egg extracts. Spliceosome inhibition resulted in the accumulation of high molecular weight centromeric transcripts, concomitant with decreased recruitment of the centromere and kinetochore proteins CENP-A, CENP-C, and NDC80 to mitotic chromosomes. In addition, blocking transcript synthesis or processing during mitosis caused accumulation of MCAK, a microtubule depolymerase, on the spindle, indicating misregulation of Aurora B. These findings suggest that co-transcriptional recruitment of the RNA processing machinery to nascent mitotic transcripts is an important step in kinetochore and spindle assembly and challenge the idea that RNA processing is globally repressed during mitosis.
AB - Transcription at the centromere of chromosomes plays an important role in kinetochore assembly in many eukaryotes, and noncoding RNAs contribute to activation of the mitotic kinase Aurora B. However, little is known about how mitotic RNA processing contributes to spindle assembly. We found that inhibition of transcription initiation or RNA splicing, but not translation, leads to spindle defects in Xenopus egg extracts. Spliceosome inhibition resulted in the accumulation of high molecular weight centromeric transcripts, concomitant with decreased recruitment of the centromere and kinetochore proteins CENP-A, CENP-C, and NDC80 to mitotic chromosomes. In addition, blocking transcript synthesis or processing during mitosis caused accumulation of MCAK, a microtubule depolymerase, on the spindle, indicating misregulation of Aurora B. These findings suggest that co-transcriptional recruitment of the RNA processing machinery to nascent mitotic transcripts is an important step in kinetochore and spindle assembly and challenge the idea that RNA processing is globally repressed during mitosis.
UR - http://www.scopus.com/inward/record.url?scp=84979998494&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84979998494&partnerID=8YFLogxK
U2 - 10.1083/jcb.201604029
DO - 10.1083/jcb.201604029
M3 - Article
C2 - 27402954
AN - SCOPUS:84979998494
SN - 0021-9525
VL - 214
SP - 133
EP - 141
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 2
ER -