Modulation of function of sodium-dependent vitamin C transporter 1 (SVCT1) by Rab8a in intestinal epithelial cells: Studies utilizing Caco-2 Cells and Rab8a knockout mice

Veedamali S. Subramanian, Sandeep B. Subramanya, Abhisek Ghosal, Jonathan S. Marchant, Akihiro Harada, Hamid M. Said

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Background: Ascorbic acid (AA) is required for normal human health and development. Human intestine expresses two sodium-dependent vitamin C transporters (hSVCT-1 and -2) that mediate cellular AA transport, with hSVCT1 targeting to the apical membrane of polarized epithelia. Studies have shown a role for the Rab8a in the apical membrane targeting of transporters in intestinal cells. Aims: The purpose of this study was to determine whether Rab8a impacts the function and/or targeting of hSVCT1, and intestinal AA uptake. Methods: We used human intestinal cells and cells from a Rab8a knockout mouse. 14C-AA uptake was performed to determine functionality. PCR and western blotting were performed to determine RNA and protein expression, respectively. Confocal imaging was performed to determine co-localization. Results: We show that hSVCT1 co-localized with Rab8a in intestinal cells. Knockdown of Rab8a lead to a significant inhibition in AA uptake and cell surface biotinylation studies revealed a lower cell surface expression of hSVCT1 in Rab8a siRNA-treated cells. Similarly, in the small intestine of a Rab8a knockout mouse, AA uptake was significantly inhibited. This effect again resulted from a decreased expression level of mSVCT1 protein, even though mRNA expression of SVCT1 was similar in intestinal cells from Rab8a knockout and wild-type litter-mates. The latter data are suggestive of enhanced lysosomal degradation of hSVCT1 protein in Rab8a-deficient cells; indeed, confocal imaging of Rab8a siRNA-treated intestinal cells revealed a strong overlap between hSVCT1-YFP and LAMP1-RFP. Conclusions: These findings show a role for Rab8a in the physiological function of hSVCT1 in intestinal epithelia.

Original languageEnglish (US)
Pages (from-to)641-649
Number of pages9
JournalDigestive Diseases and Sciences
Volume58
Issue number3
DOIs
StatePublished - Mar 2013

Bibliographical note

Funding Information:
Acknowledgments This study was supported by grants from the National Institute of Health (DK84094 to V.S.S., and DK 56061 to H.M.S., GM088790 to J.S.M.) and the Department of Veterans Affairs.

Keywords

  • Ascorbic acid
  • GTPase
  • Intestine
  • Trafficking
  • Transport
  • Vitamin C

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