Modulation of lipopolysaccharide-induced chorioamnionitis by Ureaplasma parvum in sheep

Candice C. Snyder; Katherine B. Wolfe; Tate Gisslen; Christine L. Knox; Matthew W. Kemp; Boris W. Kramer; John P. Newnham; Alan H. Jobe; Suhas G. Kallapur

doi:10.1016/j.ajog.2013.02.018

Modulation of lipopolysaccharide-induced chorioamnionitis by Ureaplasma parvum in sheep

Candice C. Snyder, Katherine B. Wolfe, Tate Gisslen, Christine L. Knox, Matthew W. Kemp, Boris W. Kramer, John P. Newnham, Alan H. Jobe, Suhas G. Kallapur

Pediatrics - Neonatology

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

Objective: Ureaplasma colonization in the setting of polymicrobial flora is common in women with chorioamnionitis, and is a risk factor for preterm delivery and neonatal morbidity. We hypothesized that Ureaplasma colonization of amniotic fluid would modulate chorioamnionitis induced by Escherichia coli lipopolysaccharide (LPS). Study Design: Sheep received intraamniotic (IA) injections of media (control) or live Ureaplasma either 7 or 70 days before delivery. Another group received IA LPS 2 days before delivery. To test for interactions, U parvum-exposed animals were challenged with IA LPS, and delivered 2 days later. All animals were delivered preterm at 125 ± 1 day of gestation. Results: Both IA Ureaplasma and LPS induced leukocyte infiltration of chorioamnion. LPS greatly increased the expression of proinflammatory cytokines and myeloperoxidase in leukocytes, while Ureaplasma alone caused modest responses. Interestingly, 7-day but not 70-day Ureaplasma exposure significantly down-regulated LPS-induced proinflammatory cytokines and myeloperoxidase expression in the chorioamnion. Conclusion: Acute (7-day) U parvum exposure can suppress LPS-induced chorioamnionitis.

Original language	English (US)
Pages (from-to)	399.e1-399.e8
Journal	American journal of obstetrics and gynecology
Volume	208
Issue number	5
DOIs	https://doi.org/10.1016/j.ajog.2013.02.018
State	Published - May 2013

Bibliographical note

Funding Information:
Supported by National Institutes of Health Grants HD 57869 (S.G.K.) and HL 97064 (A.H.J. and S.G.K.).

Keywords

endotoxin tolerance
fetal adaptation
innate immunity
preterm labor

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.ajog.2013.02.018

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title = "Modulation of lipopolysaccharide-induced chorioamnionitis by Ureaplasma parvum in sheep",

abstract = "Objective: Ureaplasma colonization in the setting of polymicrobial flora is common in women with chorioamnionitis, and is a risk factor for preterm delivery and neonatal morbidity. We hypothesized that Ureaplasma colonization of amniotic fluid would modulate chorioamnionitis induced by Escherichia coli lipopolysaccharide (LPS). Study Design: Sheep received intraamniotic (IA) injections of media (control) or live Ureaplasma either 7 or 70 days before delivery. Another group received IA LPS 2 days before delivery. To test for interactions, U parvum-exposed animals were challenged with IA LPS, and delivered 2 days later. All animals were delivered preterm at 125 ± 1 day of gestation. Results: Both IA Ureaplasma and LPS induced leukocyte infiltration of chorioamnion. LPS greatly increased the expression of proinflammatory cytokines and myeloperoxidase in leukocytes, while Ureaplasma alone caused modest responses. Interestingly, 7-day but not 70-day Ureaplasma exposure significantly down-regulated LPS-induced proinflammatory cytokines and myeloperoxidase expression in the chorioamnion. Conclusion: Acute (7-day) U parvum exposure can suppress LPS-induced chorioamnionitis.",

keywords = "endotoxin tolerance, fetal adaptation, innate immunity, preterm labor",

author = "Snyder, {Candice C.} and Wolfe, {Katherine B.} and Tate Gisslen and Knox, {Christine L.} and Kemp, {Matthew W.} and Kramer, {Boris W.} and Newnham, {John P.} and Jobe, {Alan H.} and Kallapur, {Suhas G.}",

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AU - Snyder, Candice C.

AU - Wolfe, Katherine B.

AU - Gisslen, Tate

AU - Knox, Christine L.

AU - Kemp, Matthew W.

AU - Kramer, Boris W.

AU - Newnham, John P.

AU - Jobe, Alan H.

AU - Kallapur, Suhas G.

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Modulation of lipopolysaccharide-induced chorioamnionitis by Ureaplasma parvum in sheep

Abstract

Bibliographical note

Keywords

UN SDGs

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