Modulation of lipopolysaccharide-induced chorioamnionitis by Ureaplasma parvum in sheep

Candice C. Snyder, Katherine B. Wolfe, Tate Gisslen, Christine L. Knox, Matthew W. Kemp, Boris W. Kramer, John P. Newnham, Alan H. Jobe, Suhas G. Kallapur

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Objective: Ureaplasma colonization in the setting of polymicrobial flora is common in women with chorioamnionitis, and is a risk factor for preterm delivery and neonatal morbidity. We hypothesized that Ureaplasma colonization of amniotic fluid would modulate chorioamnionitis induced by Escherichia coli lipopolysaccharide (LPS). Study Design: Sheep received intraamniotic (IA) injections of media (control) or live Ureaplasma either 7 or 70 days before delivery. Another group received IA LPS 2 days before delivery. To test for interactions, U parvum-exposed animals were challenged with IA LPS, and delivered 2 days later. All animals were delivered preterm at 125 ± 1 day of gestation. Results: Both IA Ureaplasma and LPS induced leukocyte infiltration of chorioamnion. LPS greatly increased the expression of proinflammatory cytokines and myeloperoxidase in leukocytes, while Ureaplasma alone caused modest responses. Interestingly, 7-day but not 70-day Ureaplasma exposure significantly down-regulated LPS-induced proinflammatory cytokines and myeloperoxidase expression in the chorioamnion. Conclusion: Acute (7-day) U parvum exposure can suppress LPS-induced chorioamnionitis.

Original languageEnglish (US)
Pages (from-to)399.e1-399.e8
JournalAmerican journal of obstetrics and gynecology
Volume208
Issue number5
DOIs
StatePublished - May 2013

Keywords

  • endotoxin tolerance
  • fetal adaptation
  • innate immunity
  • preterm labor

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